Part of the human host innate immune response involves the secretion of bactericidal lipids
on the skin and delivery of triglycerides into abscesses to control invading pathogens. Two S …

Specific tags with defined amino acid residues are widely used to purify or probe target
proteins. Interestingly, the tagging system occasionally results in an increase of the …

In this work, we studied the effect of the C-terminally attached poly-histidine tag (His-tag), as
well as the peculiarities of the protein purification procedure by the immobilized metal affinity …

A new Staphylococcus xylosus strain was isolated. The extracellular lipase of S. xylosus (wt-
SXL2) was purified to homogeneity from the culture medium. The specific activity of the …

We determined the effect of the His-tag on the structure, activity, stability and immobilization
of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N …

Although the use of affinity tags can greatly improve purification of expressed enzymes, the
placement of affinity tags can significantly impact the expression, solubility, and function of …

Lipases are versatile biocatalysts with many biotechnological applications and the necessity
of screening, production and characterization of new lipases from diverse microbial strains …

Due to the involvement of α-Synuclein (α-Syn) in lipid transport and its role in the normal
function and in the pathology of Parkinson disease, it is important to study first the surface …

A recombinant Serratia sp. W3 lipase (rSmL) was expressed in E. coli and purified to
homogeneity. The kinetic properties, regio-selectivity, and interfacial performances of rSmL …

The gene encoding Bacillus subtilis α-amylase was cloned into pET-21a (+). The expression
level of the recombinant enzyme was 10.7-fold higher than the expression level of the native …