LP Burakova, ES Vysotski - Applied microbiology and biotechnology, 2019 - Springer
Bright bioluminescence of ctenophores is conditioned by Ca 2+-regulated photoproteins. Although they share many properties characteristic of hydromedusan Ca 2+-regulated …
M Hematyar, V Jafarian, A Shirdel - Photochemical & Photobiological …, 2022 - Springer
We designed two mutants of photoprotein Mnemiopsin 2 (Mn2) including M52I and V144I, where the mutations were applied in the EF-hand loops I and III. Far-UV CD measurements …
A Asadi Sofilar, A Shirdel, V Jafarian… - Luminescence, 2023 - Wiley Online Library
Abstract Mnemiopsin 1 (Mn1) and Mnemiopsin 2 (Mn2) are photoproteins found in Mnemiopsis leidyi. We have tried to answer the question of whether the structural features of …
Y Ahmadi, V Jafarian, A Shirdel - Luminescence, 2024 - Wiley Online Library
We used site‐specific mutagenesis by targeting E179 and F190 on the structure of photoprotein Mnemiopsin 2 (Mn2) from Mnemiopsis leidyi. The tertiary structure of E179S …
Ca2+-regulated photoproteins (CaPhs) consist of single-chain globular proteins to which coelenterazine, a widely distributed marine luminogenic substrate (the luciferin), binds along …
To evaluate the effect of a positively charged residue at the end of the fourth helix on the function and stability of the folded state of mnemiopsin 2, Lys 89 was replaced with Ala …