Cell therapies are powerful technologies in which human cells are reprogrammed for
therapeutic applications such as killing cancer cells or replacing defective cells. The …

Synthetic gene expression in engineered cells typically depends on the host cell's limited
pool of intracellular resources, which can lead to resource competition between native and …

Here, we present a method for expressing multiple open reading frames (ORFs) from single
transcripts using the leaky scanning model of translation initiation. In this approach termed" …

Engineering synthetic regulatory circuits with precise input-output behavior—a central goal
in synthetic biology—remains encumbered by the inherent molecular complexity of cells …

The construction of synthetic gene circuits requires the rational combination of multiple
regulatory components, but predicting their behavior can be challenging due to poorly …

Conventional biological experiments often focus on in vitro assays because of the inherent
limitations when handling multiple variables in vivo, including labor-intensive and time …

Massively parallel reporter assays (MPRAs) are powerful tools for quantifying the impacts of
sequence variation on gene expression. Reading out molecular phenotypes with …

Biohybrid devices based on engineered cells interfaced with bioelectronics represent a
promising union where the strengths of each field can be synergistically combined, resulting …

Highlights•Engineering for the bioproduction of natural products is bottlenecked by
screening.•To overcome this, we explore advancements in biosensors and analytical …

Oligopool synthesis and next-generation sequencing enable the construction and
characterization of large libraries of designed genetic parts and systems. As library sizes …