The present review offers an overview of nonclassical (eg, with no pre-or in situ activation of
a carboxylic acid partner) approaches for the construction of amide bonds. The review aims …

Conspectus The coupling of an α-ketoacid and a hydroxylamine (KAHA ligation) affords
amide bonds under aqueous, acidic conditions without the need for protecting groups or …

In this article, Ser/Thr ligationon/off has been realized to enable N-to-C successive peptide
ligations using a salicylaldehyde semicarbazone (SALoff) group by in situ activation with …

Chemical protein synthesis can provide access to proteins with post-translational
modifications or site-specific labelings. Although this technology is finding increasing …

Total chemical synthesis of proteins has been rendered practical by the chemical ligation
principle: chemoselective condensation of unprotected peptide segments equipped with …

The construction of peptidic amide bonds has become a daily laboratory practice by virtue of
well-established 'coupling reagents'. Nonetheless, inherent limitations connected to these …

With the growing requirement for otherwise-difficult-to-obtain proteins, it is necessary to
develop more efficient chemical protein synthesis methods for rapid access to designed …

Total chemical synthesis of proteins allows researchers to custom design proteins without
the complex molecular biology that is required to insert non-natural amino acids or the …

Engineering at the amino acid level is key to enhancing the properties of existing proteins in
a desired manner. So far, protein engineering has been dominated by genetic approaches …

Chemical protein synthesis allows the construction of well‐defined structural variations and
facilitates the development of deeper understanding of protein structure–function …