The choice of reference genes that are stably expressed amongst treatment groups is a
crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have …

Enhanced production of high quality and healthy fry is a key target for a successful and
competitive expansion of the aquaculture industry. Although large quantities of fish larvae …

In natural fish populations, temperature increases can result in shifts in important phenotypic
traits. DNA methylation is an epigenetic mechanism mediating phenotypic changes …

Domestication of wild animals induces a set of phenotypic characteristics collectively known
as the domestication syndrome. However, how this syndrome emerges is still not clear …

Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea
bass (Dicentrarchus labrax) were identified by 2DE followed by LC‐MS/MS. From all the …

Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has
become one of the most commonly used techniques for RNA expression. To obtain more …

Despite climate-change challenges, for most aquaculture species, physiological responses
to different salinities during ambient extreme cold events remain unknown. Here, European …

The integration of genomic and environmental influences into methylation patterns to bring
about a phenotype is of central interest in developmental epigenetics, but many details are …

Extreme water temperatures related to global climate change represent a significant
challenge in terms of fish welfare and aquaculture productivity. Understanding the effect of …

To accurately quantify gene expression using quantitative PCR amplification, it is vital that
one or more ideal internal control genes are used to normalize the samples to be compared …