Emerging technologies for gene manipulation in Drosophila melanogaster
KJT Venken, HJ Bellen - Nature Reviews Genetics, 2005 - nature.com
The popularity of Drosophila melanogaster as a model for understanding eukaryotic biology
over the past 100 years has been accompanied by the development of numerous tools for …
over the past 100 years has been accompanied by the development of numerous tools for …
Transgenesis upgrades for Drosophila melanogaster
KJT Venken, HJ Bellen - 2007 - journals.biologists.com
Drosophila melanogaster is a highly attractive model system for the study of numerous
biological questions pertaining to development, genetics, cell biology, neuroscience and …
biological questions pertaining to development, genetics, cell biology, neuroscience and …
[HTML][HTML] Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
Transposable elements have been routinely used for genetic manipulation in lower
organisms, including generating transgenic animals and insertional mutagenesis. In …
organisms, including generating transgenic animals and insertional mutagenesis. In …
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes
HJ Bellen, RW Levis, G Liao, Y He, JW Carlson… - Genetics, 2004 - academic.oup.com
Abstract The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each
Drosophila gene by the insertion of a single transposable element. As part of this effort …
Drosophila gene by the insertion of a single transposable element. As part of this effort …
[HTML][HTML] Genetic manipulation of genes and cells in the nervous system of the fruit fly
Research in the fruit fly Drosophila melanogaster has led to insights in neural development,
axon guidance, ion channel function, synaptic transmission, learning and memory, diurnal …
axon guidance, ion channel function, synaptic transmission, learning and memory, diurnal …
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac
ST Thibault, MA Singer, WY Miyazaki, B Milash… - Nature …, 2004 - nature.com
With the availability of complete genome sequence for Drosophila melanogaster, one of the
next strategic goals for fly researchers is a complete gene knockout collection. The P …
next strategic goals for fly researchers is a complete gene knockout collection. The P …
Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein
microRNAs (miRNAs) are single-stranded, 21-to 23-nucleotide cellular RNAs that control the
expression of cognate target genes. Primary miRNA (pri-miRNA) transcripts are transformed …
expression of cognate target genes. Primary miRNA (pri-miRNA) transcripts are transformed …
A versatile in vivo system for directed dissection of gene expression patterns
Tissue-specific gene expression using the upstream activating sequence (UAS)–GAL4
binary system has facilitated genetic dissection of many biological processes in Drosophila …
binary system has facilitated genetic dissection of many biological processes in Drosophila …
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library
N Lowe, JS Rees, J Roote, E Ryder… - …, 2014 - journals.biologists.com
Although we now have a wealth of information on the transcription patterns of all the genes
in the Drosophila genome, much less is known about the properties of the encoded proteins …
in the Drosophila genome, much less is known about the properties of the encoded proteins …
Molecular evolutionary analysis of the widespread piggyBac transposon family and related "domesticated" sequences
Abstract piggyBac is a short inverted-repeat-type DNA transposable element originally
isolated from the genome of the moth Trichoplusia ni. It is currently the gene vector of choice …
isolated from the genome of the moth Trichoplusia ni. It is currently the gene vector of choice …