A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples

SL Nikulin, PJ Hesketh-Best, DA Mckeown, M Spivak… - PloS one, 2024 - journals.plos.org
Deformed wing virus (DWV) was first detected in dead honey bees in 1982 but has been in
honey bees for at least 300 years. Due to its high prevalence and virulence, they have been …

Automated high-throughput extraction system of sRNA and high-resolution melting quantitative PCR (HRM-qPCR) analysis for viroid detection

R Kanavedee, TN Mahamooth - Heliyon, 2023 - cell.com
A rare viroid disease, Orange Spotting (OS), has been associated with Coconut cadang-
cadang viroid variant (named OS-CCCVd) in oil palm. The low concentration of OS-CCCVd …

[HTML][HTML] Amine-modified magnetic particles: An efficient tool for enhanced RNA extraction

P Vopařilová, Z Šplíchal, P Švec, P Kulich… - Separation and …, 2025 - Elsevier
Magnetic particles are an effective tool for simple, time-saving, and labour-saving nucleic
acid extraction. In this study, we investigated the isolation of nucleic acids (NA) using 11 …

Biological and molecular characterization of citrus bent leaf viroid

M Mubeen, F Bakhtawar, Y Iftikhar, Q Shakeel, A Sajid… - Heliyon, 2024 - cell.com
Background and aim Citrus bent leaf viroid (CBLVd) is one of the emerging and widely
distributed viroids in citrus-growing areas of the world, including Pakistan. Previously …

[HTML][HTML] DAS-ELISA-based detection and RT-PCR validation for precise and timely analysis of Citrus tristeza virus infection in Nepalese citrus species

B Baral - Current Research in Biotechnology, 2024 - Elsevier
Citrus viral infections pose a substantial threat to the global citrus industry, resulting in
multitudinous economic losses annually. Timely and precise identification of citrus viruses …