OCT4 was discovered more than two decades ago as a transcription factor specific to early
embryonic development. Early studies with OCT4 were descriptive and looked at …

Single-cell RNA sequencing (RNA-seq) is a powerful tool to reveal cellular heterogeneity,
discover new cell types and characterize tumor microevolution. However, losses in cDNA …

LIF, a member of the IL6 family of cytokine, displays pleiotropic effects on various cell types
and organs. Its critical role in stem cell models (eg: murine ES, human mesenchymal cells) …

Abstract Development of a highly reproducible and sensitive single-cell RNA sequencing
(RNA-seq) method would facilitate the understanding of the biological roles and underlying …

Single-cell mRNA sequencing can uncover novel cell-to-cell heterogeneity in gene
expression levels in seemingly homogeneous populations of cells. However, these …

There is evidence that pluripotency of mouse embryonic stem (ES) cells is associated with
the activity of a network of transcription factors with Sox2, Oct4, and Nanog at the core. Using …

Exceptional genomic stability is one of the hallmarks of mouse embryonic stem (ES) cells.
However, the genes contributing to this stability remain obscure. We previously identified …

Maintaining undifferentiated mouse embryonic stem cell (mESC) culture has been a major
challenge as mESCs cultured in Leukemia Inhibitory Factor (LIF) conditions exhibit …

Polycomb repressor complexes (PRCs) are important chromatin modifiers fundamentally
implicated in pluripotency and cancer. Polycomb silencing in embryonic stem cells (ESCs) …

Embryonic stem cells (ESCs) comprise at least two populations of cells with divergent states
of pluripotency. Here, we show that epiblast stem cells (EpiSCs) also comprise two distinct …