Protease research has undergone a major expansion in the last decade, largely due to the
extremely rapid development of new technologies, such as quantitative proteomics and in …

The granzyme B-induced cell death has been traditionally viewed as a primary mechanism
that is used by cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells to eliminate …

To the Editor: Large sequence-based datasets are often scanned for conserved sequence
patterns to extract useful biological information1. Sequence logos2 have been developed to …

Effective proteome-wide strategies that distinguish the N-termini of proteins from the N-
termini of their protease cleavage products would accelerate identification of the substrates …

The ability to catalytically cleave protein substrates after synthesis is fundamental for all
forms of life. Accordingly, site-specific proteolysis is one of the most important post …

Abstract Analysis of the sequence and nature of protein N termini has many applications.
Defining the termini of proteins for proteome annotation in the Human Proteome Project is of …

Proteolysis is a major protein posttranslational modification that, by altering protein structure,
affects protein function and, by truncating the protein sequence, alters peptide signatures of …

Shotgun proteomics techniques are conceptually unbiased, but data interpretation and
follow-up experiments are often constrained by dogma, established beliefs that are accepted …

Granzyme B (GzmB) is a serine protease emerging as an important mediator of skin injury,
inflammation and repair. Found at low levels in healthy skin, GzmB is dramatically elevated …

In recent years, procedures for selecting the N-terminal peptides of proteins with analysis by
mass spectrometry have been established to characterize protease-mediated cleavage and …