It was first discovered in 1992 that P-glycoprotein (Pgp, ABCB1), an ATP binding cassette
(ABC) transporter, can transport phospholipids such as phosphatidylcholine,− ethanolamine …

Selective isotope labeling is central in NMR experiments and often allows to push the limits
on the systems investigated. It has the advantage to supply additional resolution by …

The detailed mechanism of ATP hydrolysis in ATP-binding cassette (ABC) transporters is still
not fully understood. Here, we employed 31P solid-state NMR to probe the conformational …

Nucleoside triphosphates (NTPs) are used as chemical energy source in a variety of cell
systems. Structural snapshots along the NTP hydrolysis reaction coordinate are typically …

Membrane proteins are notoriously difficult to investigate in isolation. The focus of this
chapter is the key step following extraction and purification of membrane proteins; namely …

Paramagnetic metal ions deliver structural information both in EPR and solid‐state NMR
experiments, offering a profitable synergetic approach to study bio‐macromolecules. We …

Abstract ATP-binding-cassette (ABC) transporters are molecular pumps that translocate
molecules across the cell membrane by switching between inward-facing and outward …

A number of recent advances in the field of magic-angle-spinning (MAS) solid-state NMR
have enabled its application to a range of biological systems of ever increasing complexity …

The ATP-binding cassette (ABC) transporter MsbA is an ATP-driven lipid-A flippase. It
belongs to the ABC protein superfamily whose members are characterized by conserved …

Proton‐detected 100 kHz magic‐angle‐spinning (MAS) solid‐state NMR is an emerging
analysis method for proteins with only hundreds of microgram quantities, and thus allows …