The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Improvement in intrinsic enzymatic features is in many instances a prerequisite for the
scalable applicability of many industrially important biocatalysts. To this end, various …

The relative solvent accessibility (RSA) of a residue in a protein measures the extent of
burial or exposure of that residue in the 3D structure. RSA is frequently used to describe a …

We report experimental and computational studies investigating the effects of three
osmolytes, trimethylamine N-oxide (TMAO), betaine, and glycine, on the hydrophobic …

Chemical modifications of native proteins can facilitate production of supernatural protein
functions that are not easily accessible by complementary methods relying on genetic …

This feature article provides a compilation of tools available for preparing well-defined
peptide/protein–polymer conjugates, which are defined as hybrid constructs combining (i) a …

Targeting less abundant amino acid residues on the protein surface may realize site-
selective protein modification of natural proteins. The relative hydrophobicity of tyrosine …

In antibody light chain (AL) amyloidosis, overproduced light chain (LC) fragments
accumulate as fibrils in organs and tissues of patients. In vitro, AL fibril formation is a slow …

In recent years, core/shell nanohybrids containing a nanoparticle core and a distinct
surrounding shell of polymer brushes have received extensive attention in nanoelectronics …

Ribosomally expressed proteins perform multiple, versatile, and specialized tasks
throughout Nature. In modern times, chemically modified proteins, including improved …