[HTML][HTML] Protein scaffolds in human clinics

O Cano-Garrido, N Serna, U Unzueta, E Parladé… - Biotechnology …, 2022 - Elsevier
Fundamental clinical areas such as drug delivery and regenerative medicine require
biocompatible materials as mechanically stable scaffolds or as nanoscale drug carriers …

Recombinant Proteins for Assembling as Nano-and Micro-Scale Materials for Drug Delivery: A Host Comparative Overview

JL Corchero, MTP Favaro, M Márquez-Martínez… - Pharmaceutics, 2023 - mdpi.com
By following simple protein engineering steps, recombinant proteins with promising
applications in the field of drug delivery can be assembled in the form of functional materials …

[HTML][HTML] Protein features instruct the secretion dynamics from metal-supported synthetic amyloids

E Parladé, JM Sánchez, H López-Laguna… - International journal of …, 2023 - Elsevier
Hexahistidine-tagged proteins can be clustered by divalent cations into self-containing,
dynamic protein depots at the microscale, which under physiological conditions leak …

Structural Stabilization of Clinically Oriented Oligomeric Proteins During their Transit through Synthetic Secretory Amyloids

JM Sánchez, H López‐Laguna, E Parladé… - Advanced …, 2024 - Wiley Online Library
Developing time‐sustained drug delivery systems is a main goal in innovative medicines.
Inspired by the architecture of secretory granules from the mammalian endocrine system it …

Rapid quantification of polyhydroxybutyrate polymer from single bacterial cells with mass spectrometry

SY Liang, SC Wan, YP Ho, YT Horng, PC Soo… - Analytical …, 2022 - ACS Publications
Polyhydroxyalkanoate (PHA) is one of the biocompatible and biodegradable plastics that
can be produced and accumulated as granules inside microorganisms. In this study, a new …

Hybrid Micro-/Nanoprotein Platform Provides Endocrine-like and Extracellular Matrix-like Cell Delivery of Growth Factors

H López-Laguna, PM Tsimbouri… - … Applied Materials & …, 2024 - ACS Publications
Protein materials are versatile tools in diverse biomedical fields. Among them, artificial
secretory granules (SGs), mimicking those from the endocrine system, act as mechanically …

Biofabrication of Self-Assembling Covalent Protein Nanoparticles through Histidine-Templated Cysteine Coupling

H López-Laguna, A Rueda… - ACS Sustainable …, 2023 - ACS Publications
Nanoscale protein materials show increasing applications in biotechnology and
biomedicine, addressing catalysis, drug delivery, or tissue engineering. Although protein …

Efficient Delivery of Antimicrobial Peptides in an Innovative, Slow-Release Pharmacological Formulation

N Serna, H López-Laguna, P Aceituno, M Rojas-Peña… - Pharmaceutics, 2023 - mdpi.com
Both nanostructure and multivalency enhance the biological activities of antimicrobial
peptides (AMPs), whose mechanism of action is cooperative. In addition, the efficacy of a …

Atomic force microscopy of spherical intermediates on the pathway to fibril formation of influenza A virus nuclear export protein

ON Koroleva, NV Kuzmina… - Microscopy Research …, 2024 - Wiley Online Library
The nuclear export protein of the influenza A virus (NEP) is involved in many important
processes of the virus life cycle. This makes it an attractive target for the treatment of a …

Effect of C-and N-Terminal Polyhistidine Tags on Aggregation of Influenza A Virus Nuclear Export Protein

ON Koroleva, NV Kuzmina, AP Tolstova… - Biochemistry …, 2024 - Springer
Nuclear export protein (NEP) of the influenza A virus, being one of the key components of
the virus life cycle, is a promising model for studying characteristics of formation of amyloids …