CRISPR-Cas9 is a Nobel Prize-winning robust gene-editing tool developed in the last
decade. This technique enables a stable genetic engineering method with high precision on …

The spliceosome is a staggeringly complex machine, comprising, in humans, 5 snRNAs
and> 150 proteins. We scaled haploid CRISPR-Cas9 base editing to target the entire human …

Background The transcription factor NF-κB drives neoplastic progression of many cancers
including primary brain tumors (glioblastoma [GBM]). Precise therapeutic modulation of NF …

Macrophages are critical effector cells of the immune system, and understanding genes
involved in their viability and function is essential for gaining insights into immune system …

For years, we have taken a reductionist approach to understanding gene regulation through
the study of one gene in one cell at a time. While this approach has been fruitful it is …

Pseudomonas putida KT2440 is a well-studied bacterium for the conversion of lignin-
derived aromatic compounds to bioproducts. The development of advanced genetic tools in …

MAGI1 acts as a tumor suppressor in estrogen receptor-positive (ER+) breast cancer (BC),
and its loss correlates with a more aggressive phenotype. To identify the pathways and …

Abstract Background/Objectives: Early and aggressive metastasis is a major feature of
pancreatic ductal adenocarcinoma. Understanding the processes underlying metastasis is …

Accumulating evidence has implicated impaired extracellular matrix (ECM) clearance as a
key factor in fibrotic disease. Despite decades of research elucidating the effectors of ECM …

Human and other genomes encode tens of thousands of long noncoding RNAs (lncRNAs),
the vast majority of which remain uncharacterised. High-throughput functional screening …