Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance

AM Piper, J Batovska, NOI Cogan, J Weiss… - …, 2019 - academic.oup.com
Trap-based surveillance strategies are widely used for monitoring of invasive insect species,
aiming to detect newly arrived exotic taxa as well as track the population levels of …

Future of DNA-based insect monitoring

PYS Chua, SJ Bourlat, C Ferguson, P Korlevic, L Zhao… - Trends in Genetics, 2023 - cell.com
Insects are crucial for ecosystem health but climate change and pesticide use are driving
massive insect decline. To mitigate this loss, we need new and effective monitoring …

Non‐specific amplification compromises environmental DNA metabarcoding with COI

RA Collins, J Bakker, OS Wangensteen… - Methods in Ecology …, 2019 - Wiley Online Library
Metabarcoding extra‐organismal DNA from environmental samples is now a key technique
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …

Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …

Validation of COI metabarcoding primers for terrestrial arthropods

V Elbrecht, TWA Braukmann, NV Ivanova, SWJ Prosser… - PeerJ, 2019 - peerj.com
Metabarcoding can rapidly determine the species composition of bulk samples and thus
aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that …

A practical guide to DNA metabarcoding for entomological ecologists

M Liu, LJ Clarke, SC Baker, GJ Jordan… - Ecological …, 2020 - Wiley Online Library
1. DNA metabarcoding is a cost‐effective species identification approach with great potential
to assist entomological ecologists. This review presents a practical guide to help …

To denoise or to cluster, that is not the question: optimizing pipelines for COI metabarcoding and metaphylogeography

A Antich, C Palacin, OS Wangensteen, X Turon - BMC bioinformatics, 2021 - Springer
Background The recent blooming of metabarcoding applications to biodiversity studies
comes with some relevant methodological debates. One such issue concerns the treatment …

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

F Leese, M Sander, D Buchner, V Elbrecht… - Environmental …, 2021 - Wiley Online Library
DNA metabarcoding of freshwater communities typically relies on PCR amplification of a
fragment of the mitochondrial cytochrome c oxidase I (COI) gene with degenerate primers …

Metabarcoding a diverse arthropod mock community

TWA Braukmann, NV Ivanova… - Molecular ecology …, 2019 - Wiley Online Library
Although DNA metabarcoding is an attractive approach for monitoring biodiversity, it is often
difficult to detect all the species present in a bulk sample. In particular, sequence recovery …

Environmental DNA and environmental RNA: Current and prospective applications for biological monitoring

HD Veilleux, MD Misutka, CN Glover - Science of the Total Environment, 2021 - Elsevier
Traditional environmental biomonitoring approaches have limitations in terms of species
detectability and their capacity to account for spatial and temporal variation. Furthermore, as …