[HTML][HTML] Recent advances on water-in-water emulsions in segregative systems of two water-soluble polymers

J Esquena - Current Opinion in Food Science, 2023 - Elsevier
The present paper reviews the most recent knowledge on water-in-water (W/W) emulsions
formed in aqueous two-phase systems based on incompatibility between two polymers. The …

Comparison of extracellular vesicle isolation processes for therapeutic applications

S Williams, M Fernandez-Rhodes… - Journal of Tissue …, 2023 - journals.sagepub.com
While extracellular vesicles (EVs) continue to gain interest for therapeutic applications, their
clinical translation is limited by a lack of optimal isolation methods. We sought to determine …

Downstream processing of virus‐like particles with aqueous two‐phase systems: Applications and challenges

HY Leong, XQ Fu, PL Show, SJ Yao… - Journal of Separation …, 2022 - Wiley Online Library
The advancement of recombinant virus‐like particle‐based vaccines has attracted global
attention owing to substantially safety and high efficacy in provoking a protective immunity …

Process design and optimization towards digital twins for HIV-gag VLP production in HEK293 cells, including purification

H Helgers, A Hengelbrock, A Schmidt, J Rosengarten… - Processes, 2022 - mdpi.com
Despite great efforts to develop a vaccine against human immunodeficiency virus (HIV),
which causes AIDS if untreated, no approved HIV vaccine is available to date. A promising …

The downstream bioprocess toolbox for therapeutic viral vectors

R Kilgore, A Minzoni, S Shastry, W Smith… - … of Chromatography A, 2023 - Elsevier
Viral vectors are poised to acquire a prominent position in modern medicine and
biotechnology owing to their role as delivery agents for gene therapies, oncolytic agents …

One-step salting-out extraction of bacteriophage from its infection broth of Acinetobacter baumannii

S Duan, Y Dong, Y Xu, J Yin, L Geng, Z Xiu - Journal of chromatography A, 2022 - Elsevier
Phages as a potential alternative antibiotic for multidrug-resistant bacterial infections are
receiving great attention worldwide. However, the traditional separation and purification of …

Separation and purification of enveloped and non-enveloped viruses from water samples using an aqueous two-phase system

X Hao, J Chen, M Xu, H Zheng, X Li, M Wang, T Liu - Process Biochemistry, 2022 - Elsevier
In this study, a new strategy for enriching viruses in water was developed to improve the
detection of viruses in environmental samples. The virus was enriched from water by …

A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods

S Williams, AR Jalal, MP Lewis… - Journal of Tissue …, 2023 - journals.sagepub.com
Extracellular vesicles (EVs) continue to gain interest across the scientific community for
diagnostic and therapeutic applications. As EV applications diversify, it is essential that …

Continuous and autonomous-flow separation of laccase enzyme utilizing functionalized aqueous two-phase system with computer vision control

V Sharma, JU Joo, A Mottafegh, DP Kim - Bioresource Technology, 2024 - Elsevier
Downstream processing of biomolecules, particularly therapeutic proteins and enzymes,
presents a formidable challenge due to intricate unit operations and high costs. This study …

Efficient adeno-associated virus serotype 5 capture with affinity functionalized nanofiber adsorbents

S Neto, JP Mendes, SBD Santos, A Solbrand… - … in Bioengineering and …, 2023 - frontiersin.org
Adeno-associated viruses (AAVs) are one of the most promising tools for gene therapy
applications. These vectors are purified using affinity and ion exchange chromatography …