Cryopreservation: a comprehensive overview, challenges, and future perspectives

A Parihar, A Kumar, U Panda, R Khan… - Advanced …, 2023 - Wiley Online Library
Cryopreservation is the most prevalent method of long‐term cell preservation. Effective cell
cryopreservation depends on freezing, adequate storage, and correct thawing techniques …

[PDF][PDF] Practical macromolecular cryocrystallography

JW Pflugrath - Acta Crystallographica Section F: Structural Biology …, 2015 - journals.iucr.org
Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-
ray diffraction data collection at temperatures near 100 K, where radiation damage is …

Molecular dynamics studies of polyethylene oxide and polyethylene glycol: hydrodynamic radius and shape anisotropy

H Lee, RM Venable, AD MacKerell, RW Pastor - Biophysical journal, 2008 - cell.com
A revision (C35r) to the CHARMM ether force field is shown to reproduce experimentally
observed conformational populations of dimethoxyethane. Molecular dynamics simulations …

Cryoprotectants and their usage in cryopreservation process

S Bhattacharya - … in Biomedical and Biological Sciences,-2018, 2018 - books.google.com
Cryoprotectants are basically some chemical compounds which prevent cells or tissues from
damage due to freezing. Mostly vitrification and thawing process are mostly used in …

[PDF][PDF] Effects of cryo-EM cooling on structural ensembles

LV Bock, H Grubmuller - Biophysical Journal, 2022 - cell.com
The recent revolution in cryo electron microscopy (cryo-EM) allows the determination of
structures of macromolecular complexes at atomic resolution. Cryo-EM also provides …

Cryoprotectants for freeze drying of drug nano-suspensions: effect of freezing rate

MK Lee, MY Kim, S Kim, J Lee - Journal of pharmaceutical sciences, 2009 - Elsevier
Drug nanoparticles are often prepared in a liquid medium, and a drying method such as
freeze drying is used to convert them to an oral solid dosage form. When the dried form is …

Vitrification by ultra-fast cooling at a low concentration of cryoprotectants in a quartz micro-capillary: a study using murine embryonic stem cells

X He, EYH Park, A Fowler, ML Yarmush, M Toner - Cryobiology, 2008 - Elsevier
Conventional cryopreservation protocols for slow-freezing or vitrification involve cell injury
due to ice formation/cell dehydration or toxicity of high cryoprotectant (CPA) concentrations …

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

J Wang, G Zhao, Z Zhang, X Xu, X He - Acta Biomaterialia, 2016 - Elsevier
Cryopreservation by vitrification has been recognized as a promising strategy for long-term
banking of living cells. However, the difficulty to generate a fast enough heating rate to …

[HTML][HTML] Thermostability of biological systems: fundamentals, challenges, and quantification

X He - The open biomedical engineering journal, 2011 - ncbi.nlm.nih.gov
This review examines the fundamentals and challenges in engineering/understanding the
thermostability of biological systems over a wide temperature range (from the cryogenic to …

Encapsulation of Living Cells in Small Alginate Microcapsules by Electrostatic Spraying: A Parametric Study

W Zhang, X He - 2009 - asmedigitalcollection.asme.org
A parametric study was performed to understand the effect of preparation parameters on
size, morphology, and encapsulation efficiency (ie, cells/microcapsule) of alginate …