In the past decade, membraneless assemblies known as biomolecular condensates have
been reported to play key roles in many cellular functions by compartmentalizing specific …

Many biological processes are executed and regulated through the molecular interactions of
proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the …

Many disease pathologies can be understood through the elucidation of localized
biomolecular networks, or microenvironments. To this end, enzymatic proximity labeling …

The study of protein subcellular distribution, their assembly into complexes and the set of
proteins with which they interact with is essential to our understanding of fundamental …

There are inherent limitations with traditional methods to study protein behavior or to
determine the constituency of proteins in discrete subcellular compartments. In response to …

Microscopy and mass spectrometry (MS) are complementary techniques: The former
provides spatiotemporal information in living cells, but only for a handful of recombinant …

State-of-the-art photoactivation strategies in chemical biology provide spatiotemporal control
and visualization of biological processes. However, using high-energy light (λ< 500 nm) for …

Characterizing the proteome composition of organelles and subcellular regions of living
cells can facilitate the understanding of cellular organization as well as protein interactome …

While electrophilic reagents for histidine labeling have been developed, we report an
umpolung strategy for histidine functionalization. A nucleophilic small molecule, 1-methyl-4 …

Conspectus Proximity labeling can be defined as an enzymatic “in-cell” chemical reaction
that catalyzes the proximity-dependent modification of biomolecules in live cells. Since the …