[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida

M Martin-Pascual, C Batianis, L Bruinsma… - Biotechnology …, 2021 - Elsevier
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

EM Lammens, PI Nikel, R Lavigne - Nature communications, 2020 - nature.com
Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have
unique and versatile metabolisms, offering unique opportunities for Synthetic Biology …

Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs

JS Cho, D Yang, CPS Prabowo, MR Ghiffary… - Nature …, 2023 - nature.com
Synthetic sRNAs allow knockdown of target genes at translational level, but have been
restricted to a limited number of bacteria. Here, we report the development of a broad-host …

Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories

SW Jung, J Yeom, JS Park, SM Yoo - Biotechnology Advances, 2021 - Elsevier
To overcome environmental problems caused by the use of fossil resources, microbial cell
factories have become a promising technique for the sustainable and eco-friendly …

Engineering Halomonas bluephagenesis via small regulatory RNAs

LJ Wang, XR Jiang, J Hou, CH Wang, GQ Chen - Metabolic Engineering, 2022 - Elsevier
Halomonas bluephagenesis, a robust and contamination-resistant microorganism has been
developed as a chassis for “Next Generation Industrial Biotechnology”. The non-model H …

GenoMine: a CRISPR-Cas9-based kill switch for biocontainment of Pseudomonas putida

E Asin-Garcia, M Martin-Pascual, C de Buck… - … in Bioengineering and …, 2024 - frontiersin.org
Synthetic genetic circuits have revolutionised our capacity to control cell viability by
conferring microorganisms with programmable functionalities to limit survival to specific …

Synthetic small regulatory RNAs in microbial metabolic engineering

WH Xie, HK Deng, J Hou, LJ Wang - Applied Microbiology and …, 2021 - Springer
Small regulatory RNAs (sRNAs) finely control gene expression in prokaryotes and synthetic
sRNA has become a useful high-throughput approach to tackle current challenges in …

Exploiting aerobic carboxydotrophic bacteria for industrial biotechnology

D Siebert, BJ Eikmanns, B Blombach - One-Carbon Feedstocks for …, 2021 - Springer
Aerobic carboxydotrophic bacteria are a group of microorganisms which possess the unique
trait to oxidize carbon monoxide (CO) as sole energy source with molecular oxygen (O 2) to …

Recent advances in high-throughput metabolic engineering: Generation of oligonucleotide-mediated genetic libraries

Y Li, EO Mensah, E Fordjour, J Bai, Y Yang… - Biotechnology Advances, 2022 - Elsevier
The preparation of genetic libraries is an essential step to evolve microorganisms and study
genotype-phenotype relationships by high-throughput screening/selection. As the large …

Metabolic and process engineering on the edge—Rhamnolipids are a true challenge: A review

M Filbig, S Kubicki, I Bator, R Hausmann, LM Blank… - Biosurfactants, 2023 - Elsevier
Recombinant rhamnolipid production circumvents challenges connected to the usage of
pathogenic wild-type producers such as Pseudomonas aeruginosa. In order to construct an …