Introduction: Despite tremendous advances in the application of biophysical methods in
drug discovery, the preponderance of instruments and techniques still require sophisticated …

Micro (Nano) plastics are ubiquitous in the environment and can potentially affect the toxic
effects of other chemicals, such as heavy metals. Although the interaction of micro (nano) …

Dehydrogenases are widely employed as biocatalysts for the production of optically pure
chemicals under mild conditions. Most dehydrogenases are nicotinamide cofactor (NADPH …

Enzymatic synthesis of ursodeoxycholic acid (UDCA) catalyzed by an NADH-dependent 7β-
hydroxysteroid dehydrogenase (7β-HSDH) is more economic compared with an NADPH …

Photosynthetic organisms experience wide fluctuations in light intensity and regulate light
harvesting accordingly to prevent damage from excess energy. The antenna quenching …

Analyses of intracellular NADPH concentrations are prerequisites for the design of microbial
production strains and process optimization. mBFP was described as metagenomics …

Hydroxysteroid dehydrogenases (HSDHs) catalyze the oxidation/reduction of hydroxyl/keto
groups of steroids with high regio-or stereoselectivity, playing an essential role in producing …

Abstract NAD+-dependent 7α-hydroxysteroid dehydrogenase (7α-HSDH) and NADPH-
dependent 7β-hydroxysteroid dehydrogenase (7β-HSDH) are involved in the biosynthesis of …

Switching cofactor preference of oxidoreductases from NADPH to NADH by rational
engineering, replacing the expensive cofactor NADP+ with the cheap cofactor NAD+, is a …

Heme catabolism is an important biochemical process that many bacterial pathogens utilize
to acquire iron. However, tetrapyrrole catabolites can be reactive and often require further …