A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
Cell-free protein synthesis: applications come of age
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the
growing demand for simple and efficient protein production. While used for decades as a …
growing demand for simple and efficient protein production. While used for decades as a …
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …
technology platform for high-throughput protein production and genetic part characterization …
The catalytic cycle of [FeFe] hydrogenase: A tale of two sites
JA Birrell, P Rodriguez-Macia, EJ Reijerse… - Coordination Chemistry …, 2021 - Elsevier
Ever since the discovery of hydrogenases over 90 years ago (Stephenson and Stickland,
1931), their structure and mechanism of action have been intensively investigated. Of the …
1931), their structure and mechanism of action have been intensively investigated. Of the …
Cell-free synthetic biology: thinking outside the cell
CE Hodgman, MC Jewett - Metabolic engineering, 2012 - Elsevier
Cell-free synthetic biology is emerging as a powerful approach aimed to understand,
harness, and expand the capabilities of natural biological systems without using intact cells …
harness, and expand the capabilities of natural biological systems without using intact cells …
Cell‐free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just‐add‐water cell‐free system
Biotherapeutics have many promising applications, such as anti‐cancer treatments, immune
suppression, and vaccines. However, due to their biological nature, some biotherapeutics …
suppression, and vaccines. However, due to their biological nature, some biotherapeutics …
Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Establishing a high yielding streptomyces‐based cell‐free protein synthesis system
Cell‐free protein synthesis (CFPS) has emerged as a powerful platform for applied
biotechnology and synthetic biology, with a range of applications in synthesizing proteins …
biotechnology and synthetic biology, with a range of applications in synthesizing proteins …
High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli
JM Kuchenreuther, CS Grady-Smith, AS Bingham… - PloS one, 2010 - journals.plos.org
Background The realization of hydrogenase-based technologies for renewable H2
production is presently limited by the need for scalable and high-yielding methods to supply …
production is presently limited by the need for scalable and high-yielding methods to supply …
Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract
Cell-free protein synthesis (CFPS) has become an established biotechnology tool for rapid
protein expression. Over the past few decades many advances have elevated CFPS from a …
protein expression. Over the past few decades many advances have elevated CFPS from a …