[HTML][HTML] A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
Early nonsense: mRNA decay solves a translational problem
Gene expression is highly accurate and rarely generates defective proteins. Several
mechanisms ensure this fidelity, including specialized surveillance pathways that rid the cell …
mechanisms ensure this fidelity, including specialized surveillance pathways that rid the cell …
Mammalian microRNAs predominantly act to decrease target mRNA levels
MicroRNAs (miRNAs) are endogenous∼ 22-nucleotide RNAs that mediate important gene-
regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression …
regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression …
Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution
Understanding translational control in gene expression relies on precise and
comprehensive determination of translation initiation sites (TIS) across the entire …
comprehensive determination of translation initiation sites (TIS) across the entire …
A faux 3′-UTR promotes aberrant termination and triggers nonsense- mediated mRNA decay
Nonsense-mediated messenger RNA decay (NMD) is triggered by premature translation
termination,,, but the features distinguishing premature from normal termination are …
termination,,, but the features distinguishing premature from normal termination are …
[HTML][HTML] Clarifying the translational pausing landscape in bacteria by ribosome profiling
F Mohammad, CJ Woolstenhulme, R Green… - Cell reports, 2016 - cell.com
The rate of protein synthesis varies according to the mRNA sequence in ways that affect
gene expression. Global analysis of translational pausing is now possible with ribosome …
gene expression. Global analysis of translational pausing is now possible with ribosome …
The minimum open reading frame, AUG-stop, induces boron-dependent ribosome stalling and mRNA degradation
M Tanaka, N Sotta, Y Yamazumi, Y Yamashita… - The Plant …, 2016 - academic.oup.com
Upstream open reading frames (uORFs) are often translated ahead of the main ORF of a
gene and regulate gene expression, sometimes in a condition-dependent manner, but such …
gene and regulate gene expression, sometimes in a condition-dependent manner, but such …
The mechanism of translation initiation on T ype 1 picornavirus IRES s
TR Sweeney, IS Abaeva, TV Pestova… - The EMBO journal, 2014 - embopress.org
Picornavirus T ype 1 IRES s comprise five principal domains (dII–dVI). Whereas dV binds
eIF 4 G, a conserved AUG in dVI was suggested to stimulate attachment of 43 S ribosomal …
eIF 4 G, a conserved AUG in dVI was suggested to stimulate attachment of 43 S ribosomal …
[HTML][HTML] Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain
NG Lintner, KF McClure, D Petersen… - PLoS …, 2017 - journals.plos.org
Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a key role in regulating the
levels of plasma low-density lipoprotein cholesterol (LDL-C). Here, we demonstrate that the …
levels of plasma low-density lipoprotein cholesterol (LDL-C). Here, we demonstrate that the …
[HTML][HTML] Engineered transfer RNAs for suppression of premature termination codons
Premature termination codons (PTCs) are responsible for 10–15% of all inherited disease.
PTC suppression during translation offers a promising approach to treat a variety of genetic …
PTC suppression during translation offers a promising approach to treat a variety of genetic …