Aqueous two-phase system (ATPS): an overview and advances in its applications

M Iqbal, Y Tao, S Xie, Y Zhu, D Chen, X Wang… - Biological procedures …, 2016 - Springer
Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained
an interest because of great potential for the extraction, separation, purification and …

Bioprocess optimization using design‐of‐experiments methodology

CF Mandenius, A Brundin - Biotechnology progress, 2008 - Wiley Online Library
This review surveys recent applications of design‐of‐experiments (DoE) methodology in the
development of biotechnological processes. Methods such as factorial design, response …

Method to obtain C-phycocyanin of high purity

G Patil, S Chethana, AS Sridevi… - Journal of chromatography …, 2006 - Elsevier
A new approach is made for the purification of C-phycocyanin (C-PC), which is simple and
more efficient than existing methods. The proposed method involves two steps: aqueous two …

Recovery and purification of plant-made recombinant proteins

LR Wilken, ZL Nikolov - Biotechnology advances, 2012 - Elsevier
Plants are becoming commercially acceptable for recombinant protein production for human
therapeutics, vaccine antigens, industrial enzymes, and nutraceuticals. Recently, significant …

Extraction and downstream processing of plant-derived recombinant proteins

JF Buyel, RM Twyman, R Fischer - Biotechnology advances, 2015 - Elsevier
Plants offer the tantalizing prospect of low-cost automated manufacturing processes for
biopharmaceutical proteins, but several challenges must be addressed before such goals …

[HTML][HTML] Contemporary insights into the extraction, functional properties, and therapeutic applications of plant proteins

M Navaf, KV Sunooj, B Aaliya, C Sudheesh… - Journal of Agriculture …, 2023 - Elsevier
Recently, plant proteins have received notable attention as food and medicine. Further, plant
proteins are used to produce and isolate peptides to treat and prevent diseases in humans …

Considerations for the recovery of recombinant proteins from plants

TJ Menkhaus, Y Bai, C Zhang, ZL Nikolov… - Biotechnology …, 2004 - Wiley Online Library
The past 5 years have seen the commercialization of two recombinant protein products from
transgenic plants, and many recombinant therapeutic proteins produced in plants are …

Ionic liquid aqueous two-phase systems from a pharmaceutical perspective

L McQueen, D Lai - Frontiers in Chemistry, 2019 - frontiersin.org
Aqueous Two-Phase Systems (ATPSs) have been extensively studied for their ability to
simultaneously separate and purify active pharmaceutical ingredients (APIs) and key …

Liquid–liquid extraction of proteases from fermented broth by PEG/citrate aqueous two-phase system

TS Porto, GMM e Silva, CS Porto… - … and Processing: Process …, 2008 - Elsevier
This work deals with the use of an aqueous two-phase system (ATPS) of PEG/citrate to
remove proteases from a Clostridium perfringens fermentation broth. To plan the …

Interfacial stabilization of aqueous two-phase systems: a review

C Fick, Z Khan, S Srivastava - Materials Advances, 2023 - pubs.rsc.org
Aqueous two-phase systems (ATPS) are useful in various applications, from purification and
separation of biomolecules to wastewater treatment. While they have great utility on their …