Plant protoplasts in the age of synthetic biology

I Reyna-Llorens, M Ferro-Costa… - Journal of experimental …, 2023 - academic.oup.com
Protoplasts, which are plant cells with their cell walls removed, have been used for decades
in plant research and have been instrumental in genetic transformation and the study of …

The design of synthetic gene circuits in plants: new components, old challenges

M Vazquez-Vilar, S Selma… - Journal of Experimental …, 2023 - academic.oup.com
The fascination produced by the possibility of engineering plants with augmented
capabilities has accompanied plant biotechnology since its origins. This prospect has …

Ultrathin Conductive Bithiazole‐Based Covalent Organic Framework Nanosheets for Highly Efficient Electrochemical Biosensing

W Wei, S Zhou, DD Ma, Q Li, M Ran, X Li… - Advanced Functional …, 2023 - Wiley Online Library
Covalent organic frameworks (COFs) with unique structural merits show substantial potential
in the construction of biosensors. However, high‐performance COF‐based biosensors have …

An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity

SY Park, J Qiu, S Wei, FC Peterson, J Beltrán… - Nature Chemical …, 2024 - nature.com
Plants sense abscisic acid (ABA) using chemical-induced dimerization (CID) modules,
including the receptor PYR1 and HAB1, a phosphatase inhibited by ligand-activated PYR1 …

Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought

J Lozano-Juste, L Infantes, I Garcia-Maquilon… - Science …, 2023 - science.org
Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small
molecules that act as ABA receptor agonists are promising biotechnological tools to …

Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits

D Alba Burbano, RAL Cardiff… - Proceedings of the …, 2023 - National Acad Sciences
Dynamic, multi-input gene regulatory networks (GRNs) are ubiquitous in nature. Multilayer
CRISPR-based genetic circuits hold great promise for building GRNs akin to those found in …

Synthetic transcription factor engineering for cell and gene therapy

B Bhatt, P García-Díaz, GW Foight - Trends in Biotechnology, 2024 - cell.com
Synthetic transcription factors (synTFs) that control beneficial transgene expression are an
important method to increase the safety and efficacy of cell and gene therapy. Reliance on …

Synthetic macromolecular switches for precision control of therapeutic cell functions

AP Teixeira, M Fussenegger - Nature Reviews Bioengineering, 2024 - nature.com
Cells rely on complex molecular networks to perceive and process external and internal
signals into tailored responses. These cellular abilities can be augmented and modified by …

A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

G Naseri - Nature Communications, 2023 - nature.com
Secondary natural products (NPs) are a rich source for drug discovery. However, the low
abundance of NPs makes their extraction from nature inefficient, while chemical synthesis is …

Engineering conditional protein-protein interactions for dynamic cellular control

AM Stohr, D Ma, W Chen, M Blenner - Biotechnology Advances, 2024 - Elsevier
Conditional protein-protein interactions enable dynamic regulation of cellular activity and
are an attractive approach to probe native protein interactions, improve metabolic …