Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

From 2006 to 2011, an average of 15 novel recombinant protein therapeutics have been
approved by US Food and Drug Administration (FDA) annually. In addition, the expiration of …

Transcription factors and DNA regulatory binding motifs are fundamental components of the
gene regulatory network. Here, by using genome-wide binding profiling, we show extensive …

In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that
assembles at the core promoter is required for the opening of the duplex DNA and …

In eukaryotes, the core promoter serves as a platform for the assembly of transcription
preinitiation complex (PIC) that includes TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, and RNA …

We imaged transcription in living cells using a locus-specific reporter system, which allowed
precise, single-cell kinetic measurements of promoter binding, initiation and elongation …

In a little more than 10 years, nuclear receptor (NR) coregulators (coactivators and
corepressors) have contributed to our present realization that a great level of sophistication …

Mutations that directly affect transcription by RNA polymerases rank among the most central
mediators of malignant transformation, but the frequency of new anticancer drugs that …

Nuclear receptor (NR) coregulators (coactivators and corepressors) are essential elements
in regulating nuclear receptor-mediated transcription. In a little more than a decade since …

High spatial and time resolution single-molecule fluorescence resonance energy transfer
measurements have been used to probe the structural and kinetic parameters of transfer …