Methods for profiling RNA and protein expression in a spatially resolved manner are rapidly
evolving, making it possible to comprehensively characterize cells and tissues in health and …

Explaining the diversity and complexity of protein localization is essential to fully understand
cellular architecture. Here we present cytoself, a deep-learning approach for fully self …

The nucleus displays a wide range of sizes and shapes in different species and cell types,
yet its size scaling and many of the key structural constituents that determine its shape are …

Cell proliferation is a central process in tissue development, homeostasis, and disease, yet
how proliferation is regulated in the tissue context remains poorly understood. Here, we …

Rigorous record-keeping and quality control are required to ensure the quality,
reproducibility and value of imaging data. The 4DN Initiative and BINA here propose light …

A continuing challenge in quantitative cell biology is the accurate and robust 3D
segmentation of structures of interest from fluorescence microscopy images in an …

For quality, interpretation, reproducibility and sharing value, microscopy images should be
accompanied by detailed descriptions of the conditions that were used to produce them …

Senescent cells typically have an enlarged cell size but the reason for this has not been fully
elucidated. As abnormal cell size may alter protein concentrations and cellular functionality …

Cell imaging has entered the 'Big Data'era. New technologies in light microscopy and
molecular biology have led to an explosion in high-content, dynamic and multidimensional …

Motivation Cells contain dozens of major organelles and thousands of other structures,
many of which vary extensively in their number, size, shape and spatial distribution. This …