Nanobody-based products as research and diagnostic tools
T De Meyer, S Muyldermans, A Depicker - Trends in biotechnology, 2014 - cell.com
Since the serendipitous discovery 20 years ago of bona fide camelid heavy-chain
antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies …
antibodies, their single-domain antigen-binding fragments, known as VHHs or nanobodies …
Mammalian cell protein expression for biopharmaceutical production
J Zhu - Biotechnology advances, 2012 - Elsevier
Mammalian cell expression has become the dominant recombinant protein production
system for clinical applications because of its capacity for post-translational modification and …
system for clinical applications because of its capacity for post-translational modification and …
Targeting ROS production through inhibition of NADPH oxidases
NADPH oxidases (NOXs) are transmembrane enzymes that are devoted to the production of
reactive oxygen species (ROS). In cancers, dysregulation of NOX enzymes affects ROS …
reactive oxygen species (ROS). In cancers, dysregulation of NOX enzymes affects ROS …
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody
K Kaczmarek-Hajek, J Zhang, R Kopp, A Grosche… - Elife, 2018 - elifesciences.org
The P2X7 channel is involved in the pathogenesis of various CNS diseases. An increasing
number of studies suggest its presence in neurons where its putative functions remain …
number of studies suggest its presence in neurons where its putative functions remain …
Targeting axonal guidance dependencies in glioblastoma with ROBO1 CAR T cells
Resistance to genotoxic therapies and tumor recurrence are hallmarks of glioblastoma
(GBM), an aggressive brain tumor. In this study, we investigated functional drivers of post …
(GBM), an aggressive brain tumor. In this study, we investigated functional drivers of post …
High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells
Background The demand of monospecific high affinity binding reagents, particularly
monoclonal antibodies, has been steadily increasing over the last years. Enhanced …
monoclonal antibodies, has been steadily increasing over the last years. Enhanced …
Nanobodies that block gating of the P2X7 ion channel ameliorate inflammation
W Danquah, C Meyer-Schwesinger, B Rissiek… - Science translational …, 2016 - science.org
Ion channels are desirable therapeutic targets, yet ion channel–directed drugs with high
selectivity and few side effects are still needed. Unlike small-molecule inhibitors, antibodies …
selectivity and few side effects are still needed. Unlike small-molecule inhibitors, antibodies …
Engineered multivalent nanobodies potently and broadly neutralize SARS‐CoV‐2 variants
The COVID‐19 pandemic continues to be a severe threat to human health, especially due to
current and emerging SARS‐CoV‐2 variants with potential to escape humoral immunity …
current and emerging SARS‐CoV‐2 variants with potential to escape humoral immunity …
Production of α2, 6-sialylated IgG1 in CHO cells
C Raymond, A Robotham, M Spearman, M Butler… - MAbs, 2015 - Taylor & Francis
The presence of α2, 6-sialic acids on the Fc N-glycan provides anti-inflammatory properties
to the IgGs through a mechanism that remains unclear. Fc-sialylated IgGs are rare in …
to the IgGs through a mechanism that remains unclear. Fc-sialylated IgGs are rare in …
The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal …
B Liu, M Spearman, J Doering, E Lattová… - Journal of …, 2014 - Elsevier
The glycosylation pattern of a chimeric heavy chain antibody (EG2) produced from CHO
cells was affected by the glucose concentration (0–25 mM) of cultures established at high …
cells was affected by the glucose concentration (0–25 mM) of cultures established at high …