A pragmatic guide to enrichment strategies for mass spectrometry–based glycoproteomics

NM Riley, CR Bertozzi, SJ Pitteri - Molecular & Cellular Proteomics, 2021 - ASBMB
Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

Chemical glycoproteomics

KK Palaniappan, CR Bertozzi - Chemical reviews, 2016 - ACS Publications
Chemical tools have accelerated progress in glycoscience, reducing experimental barriers
to studying protein glycosylation, the most widespread and complex form of posttranslational …

Proteomic techniques for characterisation of mesenchymal stem cell secretome

HK Skalnikova - Biochimie, 2013 - Elsevier
Mesenchymal stem cells (MSCs) are multipotent cells with a substantial potential in human
regenerative medicine due to their ability to migrate to sites of injury, capability to suppress …

Recent advances in glycoproteomic analysis by mass spectrometry

S Suttapitugsakul, F Sun, R Wu - Analytical chemistry, 2019 - ACS Publications
Glycosylation is one of the most common protein modifications and is essential for cells. This
modification is exceptionally complex because glycans are highly diverse and can be …

Highly coordinated proteome dynamics during reprogramming of somatic cells to pluripotency

J Hansson, MR Rafiee, S Reiland, JM Polo, J Gehring… - Cell reports, 2012 - cell.com
Generation of induced pluripotent stem cells (iPSCs) is a process whose mechanistic
underpinnings are only beginning to emerge. Here, we applied in-depth quantitative …

Defining the cell surface cysteinome using two-step enrichment proteomics

T Yan, LM Boatner, L Cui, PJ Tontonoz, KM Backus - JACS Au, 2023 - ACS Publications
The plasma membrane proteome is a rich resource of functionally important and
therapeutically relevant protein targets. Distinguished by high hydrophobicity, heavy …

Recent advances in mass spectrometry (MS)-based glycoproteomics in complex biological samples

Z Chen, J Huang, L Li - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Protein glycosylation plays a key role in various biological processes and disease-related
pathological progression. Mass spectrometry (MS)-based glycoproteomics is a powerful …

Integral membrane proteins in proteomics. How to break open the black box?

O Vit, J Petrak - Journal of Proteomics, 2017 - Elsevier
Integral membrane proteins (IMPs) are coded by 20–30% of human genes and execute
important functions–transmembrane transport, signal transduction, cell-cell communication …

[HTML][HTML] High efficiency differentiation of human pluripotent stem cells to cardiomyocytes and characterization by flow cytometry

S Bhattacharya, PW Burridge, EM Kropp… - Journal of …, 2014 - pmc.ncbi.nlm.nih.gov
There is an urgent need to develop approaches for repairing the damaged heart,
discovering new therapeutic drugs that do not have toxic effects on the heart, and improving …

Exoproteomics: exploring the world around biological systems

J Armengaud, JA Christie-Oleza, G Clair… - Expert Review of …, 2012 - Taylor & Francis
The term 'exoproteome'describes the protein content that can be found in the extracellular
proximity of a given biological system. These proteins arise from cellular secretion, other …