The advent of large genolne sequencing projects has changed the scale of biology. Over a
relatively short period of tinle, we have witnessed the elucidation of the conlplete nucleotide …

Abstract Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry (MS) is currently the workhorse …

Thoroughly revised and reorganized, the second edition of Interfacial Forces in Aqueous
Media examines the role of polar interfacial and noncovalent interactions among biological …

The focusing positions in narrow range immobilized pH gradients of 29 polypeptides of
known amino acid sequence were determined under denaturing conditions. The isoelectric …

A highly reproducible, commercial and nonlinear, wide‐range immobilized pH gradient
(IPG) was used to generate two‐dimensional (2‐D) gel maps of [35S] methionine‐labeled …

This fifth edition of the successful, long-selling classic has been completely revised and
expanded, omitting some topics on obsolete DNA electrophoresis, but now with a …

Gel-based proteomics is one of the most versatile methods for fractionating protein
complexes. Among these methods, two dimensional-polyacrylamide gel electrophoresis (2 …

This study focuses on the specific problems of protein extraction from recalcitrant plant
tissues and evaluates several methods to bypass them. Sample preparation is a critical step …

Immobilized pH gradients (IPG) were introduced in 1982 [1]. An immediate advance of
isoelectric focusing (IEF) with IPG was the practical feasibility of setting up very narrow pH …

Proteomes are complex—much more so than genomes or transcriptomes. Thus, simplifying
their analysis does not simplify the issue. Proteomes are of proteoforms, not canonical …