Evaluation of Sorghum [Sorghum bicolor (L.)] Reference Genes in Various Tissues and under Abiotic Stress Conditions for Quantitative Real-Time PCR Data …

P Sudhakar Reddy, D Srinivas Reddy… - Frontiers in plant …, 2016 - frontiersin.org
Accurate and reliable gene expression data from qPCR depends on stable reference gene
expression for potential gene functional analyses. In this study, 15 reference genes were …

Identification of suitable reference genes for gene expression normalization in qRT-PCR analysis in watermelon

Q Kong, J Yuan, L Gao, S Zhao, W Jiang, Y Huang… - PloS one, 2014 - journals.plos.org
Watermelon is one of the major Cucurbitaceae crops and the recent availability of genome
sequence greatly facilitates the fundamental researches on it. Quantitative real-time reverse …

Expression of malic enzyme reveals subcellular carbon partitioning for storage reserve production in soybeans

SA Morley, F Ma, M Alazem, C Frankfater, H Yi… - New …, 2023 - Wiley Online Library
Central metabolism produces amino and fatty acids for protein and lipids that establish seed
value. Biosynthesis of storage reserves occurs in multiple organelles that exchange central …

Peanuts that keep aflatoxin at bay: a threshold that matters

KK Sharma, A Pothana, K Prasad… - Plant biotechnology …, 2018 - Wiley Online Library
Aflatoxin contamination in peanuts poses major challenges for vulnerable populations of
sub‐Saharan Africa and South Asia. Developing peanut varieties to combat preharvest …

Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya

C Chen, J Wu, Q Hua, N Tel-Zur, F Xie, Z Zhang… - Plant Methods, 2019 - Springer
Background A suitable reference gene is an important prerequisite for guarantying accurate
and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no …

Identification and expression analysis of WRKY gene family under drought stress in peanut (Arachis hypogaea L.)

N Zhao, M He, L Li, S Cui, M Hou, L Wang, G Mu… - PLoS …, 2020 - journals.plos.org
WRKY transcription factors play crucial roles in regulation mechanism leading to the
adaption of plants to the complex environment. In this study, AhWRKY family was …

Systematic selection and validation of suitable reference genes for quantitative real-time PCR normalization studies of gene expression in Nitraria tangutorum

B Wang, H Duan, P Chong, S Su, L Shan, D Yi… - Scientific Reports, 2020 - nature.com
Suitable reference genes can be used to calibrate the error in quantitative real-time PCR
(qPCR) experiments, making the results more credible. However, there are no reference …

Selection and Validation of Housekeeping Genes as Reference for Gene Expression Studies in Pigeonpea (Cajanus cajan) under Heat and Salt Stress Conditions

P Sinha, RK Saxena, VK Singh… - Frontiers in Plant …, 2015 - frontiersin.org
To identify stable housekeeping genes as a reference for expression analysis under heat
and salt stress conditions in pigeonpea, the relative expression variation for 10 commonly …

Evaluation and Validation of Housekeeping Genes as Reference for Gene Expression Studies in Pigeonpea (Cajanus cajan) Under Drought Stress Conditions

P Sinha, VK Singh, V Suryanarayana, L Krishnamurthy… - PloS one, 2015 - journals.plos.org
Gene expression analysis using quantitative real-time PCR (qRT-PCR) is a very sensitive
technique and its sensitivity depends on the stable performance of reference gene (s) used …

Arachis hypogaea gene expression atlas for fastigiata subspecies of cultivated groundnut to accelerate functional and translational genomics applications

P Sinha, P Bajaj, LT Pazhamala… - Plant Biotechnology …, 2020 - Wiley Online Library
Spatio‐temporal and developmental stage‐specific transcriptome analysis plays a crucial
role in systems biology‐based improvement of any species. In this context, we report here …