Protein Engineering and High‐Throughput Screening by Yeast Surface Display: Survey of Current Methods

J Lopez-Morales, R Vanella, EA Appelt… - Small …, 2023 - Wiley Online Library
Yeast surface display (YSD) is a powerful tool in biotechnology that links genotype to
phenotype. In this review, the latest advancements in protein engineering and high …

Direct Comparison of Lysine versus Site‐Specific Protein Surface Immobilization in Single‐Molecule Mechanical Assays

H Liu, Z Liu, M Sá Santos… - Angewandte Chemie …, 2023 - Wiley Online Library
Single‐molecule force spectroscopy (SMFS) is powerful for studying folding states and
mechanical properties of proteins, however, it requires protein immobilization onto force …

Two molecule force spectroscopy on ligand–receptor interactions

J Zuo, H Chen, H Li - Nanoscale, 2023 - pubs.rsc.org
Many biological processes involve the rupture of multiple ligand–receptors or multivalent
ligand–receptors. It is challenging to study the rupture of such parallelly arranged multiple …

Engineering the Mechanical Stability of a Therapeutic Complex between Affibody and Programmed Death-Ligand 1 by Anchor Point Selection

B Yang, DEB Gomes, Z Liu, MS Santos, J Li… - ACS …, 2024 - ACS Publications
Protein–protein complexes can vary in mechanical stability depending on the direction from
which force is applied. Here, we investigated the mechanical stability of a complex between …

Titrating avidity of yeast-displayed proteins using a transcriptional regulator

J Lopez-Morales, R Vanella, G Kovacevic… - ACS Synthetic …, 2023 - ACS Publications
Yeast surface display is a valuable tool for protein engineering and directed evolution;
however, significant variability in the copy number (ie, avidity) of displayed variants on the …

Engineering the Mechanical Stability of a Therapeutic Affibody/PD-L1 Complex by Anchor Point Selection

B Yang, DEB Gomes, Z Liu, MS Santos, J Li… - …, 2024 - pmc.ncbi.nlm.nih.gov
Protein-protein complexes can vary in mechanical stability depending on the direction from
which force is applied. Here we investigated the anisotropic mechanical stability of a …

Anchor Point Engineering on Anticalin Scaffolds for Enhanced Particle Adhesion to CTLA-4 Under Shear Stress

Y Sun, J Li, R Vanella, H Liu, Z Liu, M Nash - bioRxiv, 2024 - biorxiv.org
Achieving high binding strength and efficient delivery of molecular cargo to tumor cells is a
significant challenge in cancer immunotherapy. Current research has focused on obtaining …

Rationally designing dynamic protein cross-linked hydrogels across length scales

Z Walsh-Korb - Bioinspiration, Biomimetics, and Bioreplication …, 2024 - spiedigitallibrary.org
Nature's exquisite designs are a constant source of inspiration for materials scientists, yet
our ability to replicate biological interactions in engineered systems is often limited …