In last years, the main studied microbial sources of natural blue pigments have been the
eukaryotic algae, Rhodophytes and Cryptophytes, and the cyanobacterium Arthrospira …

Highlights•Kinetic metabolic modeling could enable rational bioengineering and dynamic
understanding of metabolism.•We discuss experimental and computational approaches to …

The application of rational design in reallocating metabolic flux to overproduce desired
chemicals is always restricted by the native regulatory network. Here, we demonstrated that …

ABSTRACT A powerful approach for the optimization of industrial bioprocesses is to perform
detailed simulations integrating large‐scale computational fluid dynamics (CFD) and cellular …

Metabolomics aims to address what and how regulatory mechanisms are coordinated to
achieve flux optimality, different metabolic objectives as well as appropriate adaptations to …

In a 54 m3 large‐scale penicillin fermentor, the cells experience substrate gradient cycles at
the timescales of global mixing time about 20–40 s. Here, we used an intermittent feeding …

In large‐scale bioreactors, there is often insufficient mixing and as a consequence, cells
experience uneven substrate and oxygen levels that influence product formation. In this …

In the present work, by performing chemostat experiments at 400 and 600 RPM, two typical
power inputs representative of industrial penicillin fermentation (P/V, 1.00 kW/m3 in more …

Targeted, quantitative metabolomics can, in principle, provide precise information on
intracellular metabolite levels, which can be applied to accurate modeling of intracellular …

Due to insufficient mass transfer and mixing issues, cells in the industrial-scale bioreactor
are often forced to experience glucose feast/famine cycles, mostly resulting in reduced …