Although bulk protein turnover has been measured with the use of stable isotope labeled
tracers for over half a century, it is only recently that the same approach has become …

Protein degradation is a crucial regulatory process in maintaining cellular proteostasis. The
selective degradation of intracellular proteins controls diverse cellular and biochemical …

Ataxin-3 is a deubiquitinating enzyme and the affected protein in the neurodegenerative
disorder Machado–Joseph disease (MJD). The ATXN3 gene is alternatively spliced …

Ocular coloboma results from abnormal embryonic development and is often associated
with additional ocular and systemic features. Coloboma is a highly heterogeneous disorder …

Autophagy is an intracellular degradation mechanism in response to nutrient starvation. Via
autophagy, some nonessential cellular constituents are degraded in a lysosome-dependent …

We have developed a method combining microinjection and automated fluorescence
microscopy to continuously assess the degradation rate, subcellular localization and …

Abstract DJ-1/PARK7 mutations are linked with familial forms of early-onset Parkinson's
disease (PD). We have studied the degradation of untagged DJ-1 wild type (WT) and …

Abstract αSynuclein (αSyn) misfolding and aggregation frequently precedes neuronal loss
associated with Parkinson's Disease (PD) and other Synucleinopathies. The progressive …

The closely related T-box transcription factors TBX2 and TBX3 are frequently overexpressed
in melanoma and various types of human cancers, in particular, breast cancer. The …

Protein synthesis inhibitors are commonly used for measuring protein degradation rates, but
may cause cytotoxicity via direct or indirect mechanisms. This study aimed to identify …