Today, reverse phase liquid chromatography (RPLC) analysis of proteins is almost
exclusively performed on conventional columns (100–150 mm) in gradient elution mode …

There has been a significant increase in the use of hydrophilic interaction liquid
chromatography (HILIC) to separate oligonucleotides. This rise in the use of HILIC has …

Oligonucleotide characterization is a rapidly advancing field in the biopharmaceutical
industry. Understanding critical quality attributes, such as intact mass and impurities …

Therapeutic oligonucleotides (ONs) are a new generation of drugs that have captured the
attention of the pharmaceutical industry over the past decade, as evidenced by the FDA …

With this work, we present a comprehensive tutorial for the analysis of oligonucleotides
(ONs, 5 to 100 mer) using ion-pair reversed-phase liquid chromatography (IP-RPLC) on …

Height equivalent to theoretical plate (H) equations, such as the van Deemter or Knox-
Saleem equations, and other efficiency vs. linear velocity equations (u), provide kinetic …

In recent years, many nucleic acid-based pharmaceuticals have been approved and entered
the market, and even a larger number are in late stage clinical trials. Conventional …

The rapid development of mRNA-based therapeutics, especially post-COVID-19, has
necessitated the precise characterization of mRNA quality attributes, including sequence …

Ion-pair reversed-phase liquid chromatography (IP-RPLC) is clearly recognized as the gold
standard for analyzing therapeutic oligonucleotides (ONs). Recent studies have shown that …

Oligonucleotides constitute an emerging and highly complex bioanalytical challenge and it
is becoming increasingly clear that 1D methodologies are unable to fully resolve all possible …