[HTML][HTML] Integrated intracellular organization and its variations in human iPS cells

MP Viana, J Chen, TA Knijnenburg, R Vasan, C Yan… - Nature, 2023 - nature.com
Understanding how a subset of expressed genes dictates cellular phenotype is a
considerable challenge owing to the large numbers of molecules involved, their …

[HTML][HTML] Focus: Cell Fate: Advances in Regulating Cellular Behavior Using Micropatterns

Y Li, W Jiang, X Zhou, Y Long, Y Sun… - The Yale Journal of …, 2023 - ncbi.nlm.nih.gov
Micropatterns, characterized as distinct physical microstructures or chemical adhesion
matrices on substance surfaces, have emerged as a powerful tool for manipulating cellular …

Symmetry Breaking of Human Pluripotent Stem Cells (hPSCs) in Micropattern Generates a Polarized Spinal Cord‐Like Organoid (pSCO) with Dorsoventral …

K Seo, S Cho, H Shin, A Shin, JH Lee… - Advanced …, 2023 - Wiley Online Library
Axis formation and related spatial patterning are initiated by symmetry breaking during
development. A geometrically confined culture of human pluripotent stem cells (hPSCs) …

Cell behavioral dynamics as a cue in optimizing culture stabilization in the bioprocessing of pluripotent stem cells

N Thanuthanakhun, MH Kim, M Kino-Oka - Bioengineering, 2022 - mdpi.com
Pluripotent stem cells (PSCs) are important for future regenerative medicine therapies.
However, in the production of PSCs and derivatives, the control of culture-induced …

High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies

AJ Asmar, ZA Benson, AP Peskin, J Chalfoun, M Simon… - Plos one, 2024 - journals.plos.org
To facilitate the characterization of unlabeled induced pluripotent stem cells (iPSCs) during
culture and expansion, we developed an AI pipeline for nuclear segmentation and mitosis …

Micro-patterned culture of iPSC-derived alveolar and airway cells distinguishes SARS-CoV-2 variants

A Masui, R Hashimoto, Y Matsumura, T Yamamoto… - Stem cell reports, 2024 - cell.com
The emergence of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) variants
necessitated a rapid evaluation system for their pathogenesis. Lung epithelial cells are their …

Stable and efficient generation of functional iPSC-derived neural progenitor cell rosettes through regulation of collective cell-cell behavior

MH Kim, N Thanuthanakhun… - Frontiers in Bioengineering …, 2024 - frontiersin.org
Although the potential of stem cells to differentiate into several cell types has shown promise
in regenerative medicine, low differentiation efficiency and poor reproducibility significantly …

Novel strategy to improve hepatocyte differentiation stability through synchronized behavior‐driven mechanical memory of iPSCs

MH Kim, N Thanuthanakhun… - Biotechnology and …, 2023 - Wiley Online Library
Cellular homeostasis is assumed to be regulated by the coordination of dynamic behaviors.
Lack of efficient methods for synchronizing large quantities of cells makes studying cell …

Arp2/3 complex activity enables nuclear YAP for naïve pluripotency of human embryonic stem cells

NP Meyer, T Singh, ML Kutys, TG Nystul, DL Barber - Elife, 2024 - elifesciences.org
Our understanding of the transitions of human embryonic stem cells (hESCs) between
distinct stages of pluripotency relies predominantly on regulation by transcriptional and …

Suicide gene‐enabled cell therapy: A novel approach to scalable human pluripotent stem cell quality control

E Gysel, L Larijani, MS Kallos, RJ Krawetz - BioEssays, 2023 - Wiley Online Library
There are an increasing number of cell therapy approaches being studied and employed
world‐wide. An emerging area in this field is the use of human pluripotent stem cell (hPSC) …