Since its discovery in the 1970s, the T7 RNA polymerase (T7 RNAP) transcription system
has been applied extensively as an effective tool in molecular biology because of its robust …

Faithful transmission of genetic material is essential for the survival of all organisms.
Eukaryotic chromosome segregation is driven by the kinetochore that assembles onto …

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the
ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most …

One of the first steps in understanding a protein's function is to determine its localization;
however, the methods for localizing proteins in some systems have not kept pace with the …

The kinetochore is the macromolecular protein complex that directs chromosome
segregation in eukaryotes. It has been widely assumed that the core kinetochore consists of …

The protozoan parasites Trypanosoma brucei spp. cause important human and livestock
diseases in sub-Saharan Africa. In mammalian blood, two developmental forms of the …

We have generated a high-confidence mitochondrial proteome (MitoTag) of the
Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a …

Variant surface glycoprotein (VSG) coats bloodstream form Trypanosoma brucei parasites,
and monoallelic VSG expression underpins the antigenic variation necessary for …

Trypanosome parasites control their virulence and spread by using quorum sensing (QS) to
generate transmissible" stumpy forms" in their host bloodstream. However, the QS signal" …

The co-infection cases involving dixenous Leishmania spp.(mostly of the L. donovani
complex) and presumably monoxenous trypanosomatids in immunocompromised …