Precursor messenger RNA (pre-mRNA) splicing is an essential step in the flow of
information from DNA to protein in all eukaryotes. Research over the past four decades has …

Hereditary degenerations of the human retina are genetically heterogeneous, with well over
100 genes implicated so far. This Seminar focuses on the subset of diseases called retinitis …

Mechanistic understanding of pre-mRNA splicing requires detailed structural information on
various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) …

Precursor mRNA (pre-mRNA) splicing proceeds by two consecutive transesterification
reactions via a lariat–intron intermediate. Here we present the 3.8 Å cryo-electron …

Pre-messenger RNA (pre-mRNA) splicing is a central step in gene expression. Lying
between transcription and protein synthesis, pre-mRNA splicing removes sequences …

Splicing of the precursor messenger RNA, involving intron removal and exon ligation, is
mediated by the spliceosome. Together with biochemical and genetic investigations of the …

Each cycle of precursor messenger RNA (pre-mRNA) splicing comprises two sequential
reactions, first freeing the 5′ exon and generating an intron lariat–3′ exon and then …

We provide direct evidence that pre-mRNA splicing alters mRNP protein composition. Using
a novel in vitro cross-linking approach, we detected several proteins that associate with …

In order to assess the role of Prp22 in yeast pre‐mRNA splicing, we have purified the 130
kDa Prp22 protein and developed an in vitro depletion/reconstitution assay. We show that …

RNA helicases represent a large family of proteins that have been detected in almost all
biological systems where RNA plays a central role. They are ubiquitously distributed over a …