Droplet-based microfluidics

T Moragues, D Arguijo, T Beneyton, C Modavi… - Nature Reviews …, 2023 - nature.com
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Systems integration for global sustainability

J Liu, H Mooney, V Hull, SJ Davis, J Gaskell, T Hertel… - Science, 2015 - science.org
BACKGROUND Many key global sustainability challenges are closely intertwined
(examples are provided in the figure). These challenges include air pollution, biodiversity …

Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

M Weiss, JP Frohnmayer, LT Benk, B Haller… - Nature materials, 2018 - nature.com
Compartments for the spatially and temporally controlled assembly of biological processes
are essential towards cellular life. Synthetic mimics of cellular compartments based on lipid …

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

DJ Collins, A Neild, A DeMello, AQ Liu, Y Ai - Lab on a Chip, 2015 - pubs.rsc.org
There is a recognized and growing need for rapid and efficient cell assays, where the size of
microfluidic devices lend themselves to the manipulation of cellular populations down to the …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Droplet based microfluidics

R Seemann, M Brinkmann, T Pfohl… - Reports on progress …, 2011 - iopscience.iop.org
Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining
soft matter physics, biochemistry and microsystems engineering. Its applications range from …

Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications

D Mark, S Haeberle, G Roth, F Von Stetten… - Microfluidics based …, 2010 - Springer
This review summarizes recent developments in microfluidic platform approaches. In
contrast to isolated application-specific solutions, a microfluidic platform provides a set of …

High-throughput screening by droplet microfluidics: Perspective into key challenges and future prospects

EM Payne, DA Holland-Moritz, S Sun, RT Kennedy - Lab on a Chip, 2020 - pubs.rsc.org
In two decades of development, impressive strides have been made for automating basic
laboratory operations in droplet-based microfluidics, allowing the emergence of a new form …

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

JJ Agresti, E Antipov, AR Abate, K Ahn… - Proceedings of the …, 2010 - National Acad Sciences
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving
ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively …