Chromosome Duplication in Saccharomyces cerevisiae
SP Bell, K Labib - Genetics, 2016 - academic.oup.com
The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …
Rescuing stalled or damaged replication forks
JTP Yeeles, J Poli, KJ Marians… - Cold Spring Harbor …, 2013 - cshperspectives.cshlp.org
In recent years, an increasing number of studies have shown that prokaryotes and
eukaryotes are armed with sophisticated mechanisms to restart stalled or collapsed …
eukaryotes are armed with sophisticated mechanisms to restart stalled or collapsed …
The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
A Yesbolatova, Y Saito, N Kitamoto… - Nature …, 2020 - nature.com
Protein knockdown using the auxin-inducible degron (AID) technology is useful to study
protein function in living cells because it induces rapid depletion, which makes it possible to …
protein function in living cells because it induces rapid depletion, which makes it possible to …
DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans
Assembly of the CMG (CDC-45–MCM-2-7–GINS) helicase is the key regulated step during
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …
Regulated eukaryotic DNA replication origin firing with purified proteins
JTP Yeeles, TD Deegan, A Janska, A Early, JFX Diffley - Nature, 2015 - nature.com
Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated
to promote precise genome duplication in every cell cycle. To accomplish this, initiation is …
to promote precise genome duplication in every cell cycle. To accomplish this, initiation is …
An expanded tool kit for the auxin‐inducible degron system in budding yeast
M Morawska, HD Ulrich - Yeast, 2013 - Wiley Online Library
Fusion of inducible degradation signals, so‐called degrons, to cellular proteins is an elegant
method of controlling protein levels in vivo. Recently, a degron system relying on the plant …
method of controlling protein levels in vivo. Recently, a degron system relying on the plant …
Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation
The eukaryotic CMG (Cdc45, Mcm2–7, GINS) helicase consists of the Mcm2–7 hexameric
ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric …
ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric …
MCM: one ring to rule them all
TD Deegan, JFX Diffley - Current opinion in structural biology, 2016 - Elsevier
Highlights•The eukaryotic MCM replicative helicase is tightly regulated during the cell
cycle.•Conformational changes in MCM underpin the different stages of chromosome …
cycle.•Conformational changes in MCM underpin the different stages of chromosome …
Replication fork activation is enabled by a single-stranded DNA gate in CMG helicase
MR Wasserman, GD Schauer, ME O'Donnell, S Liu - Cell, 2019 - cell.com
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds) DNA
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …
Helicase activation and establishment of replication forks at chromosomal origins of replication
Many replication proteins assemble on the pre-RC-formed replication origins and constitute
the pre-initiation complex (pre-IC). This complex formation facilitates the conversion of Mcm2 …
the pre-initiation complex (pre-IC). This complex formation facilitates the conversion of Mcm2 …