In vitro cell cultures are crucial research tools for modeling human development and
diseases. Although the conventional monolayer cell cultures have been widely used in the …

Organoid systems leverage the self-organizing properties of stem cells to create diverse
multi-cellular tissue proxies. Most organoid models only represent single or partial …

Human pluripotent stem cells have emerged as a promising in vitro model system for
studying the brain. Two-dimensional and three-dimensional cell culture paradigms have …

Direct lineage reprogramming represents a remarkable conversion of cellular and
transcriptome states,,. However, the intermediate stages through which individual cells …

Human pluripotent stem cells (PSCs) are a promising source of cells for applications in
regenerative medicine. Directed differentiation of PSCs into specialized cells such as spinal …

Human pluripotent stem cells (hPSCs) have opened new opportunities for understanding
human development, modelling disease processes and developing new therapeutics …

Human cell reprogramming technologies offer access to live human neurons from patients
and provide a new alternative for modeling neurological disorders in vitro. Neural electrical …

Current neural induction protocols for human embryonic stem (hES) cells rely on embryoid
body formation, stromal feeder co-culture or selective survival conditions. Each strategy has …

Human pluripotent stem cells (hPSCs) provide a model to study early neural development,
model pathological processes, and develop therapeutics. The generation of functionally …

Abstract Specification of cell identity during development depends on exposure of cells to
sequences of extrinsic cues delivered at precise times and concentrations. Identification of …