During the last 30years the scope of biocatalysis has been expanding due to the advances
in several technological fields. Diverse techniques as structural enzyme improvement (eg …

▪ Abstract Understanding the action of enzymes on an atomistic level is one of the important
aims of modern biophysics. This review describes the state of the art in addressing this …

Recent advances in the study of the catalytic properties of acetylcholinesterases have been
reviewed. The main biological function of this enzyme is the fast termination of impulse …

Almost all proteins contain charged amino acids. While the function in catalysis or binding of
individual charges in the active site can often be identified, it is less clear how to assign …

Some half-century ago Pauling (1, 2) proposed that the lowering of the activation energy in
enzyme catalysis stems from the enzyme's affinity for the transition state exceeding it's …

▪ Abstract Enzymes are called upon to differ greatly in the difficulty of the tasks that they
perform. The catalytic proficiency of an enzyme can be evaluated by comparing the second …

With many genomes sequenced, a pressing challenge in biology is predicting the function of
the proteins that the genes encode. When proteins are unrelated to others of known activity …

Comparative analysis of the sequences of enzymes encoded in a variety of prokaryotic and
eukaryotic genomes reveals convergence and divergence at several levels. Functional …

Phosphoserine phosphatase (PSP) is a member of a large class of enzymes that catalyze
phosphoester hydrolysis using a phosphoaspartate–enzyme intermediate. PSP is a likely …

The origins of enzyme catalysis have been attributed to both transition-state stabilization as
well as ground-state destabilization of the substrate. For the latter paradigm, the enzyme …