An ex vivo ovulation system enables the discovery of novel ovulatory pathways and nonhormonal contraceptive candidates

J Zhang, BA Goods, P Pattarawat, Y Wang… - Biology of …, 2023 - academic.oup.com
Ovulation is an integral part of women's menstrual cycle and fertility. Understanding the
mechanisms of ovulation has broad implications for the treatment of anovulatory diseases …

[HTML][HTML] Three-Dimensionally Printed Agarose Micromold Supports Scaffold-Free Mouse Ex Vivo Follicle Growth, Ovulation, and Luteinization

EJ Zaniker, PH Hashim, S Gauthier, JA Ankrum… - Bioengineering, 2024 - mdpi.com
Ex vivo follicle growth is an essential tool, enabling interrogation of folliculogenesis,
ovulation, and luteinization. Though significant advancements have been made, existing …

Follicle-intrinsic and spatially distinct molecular programs drive follicle rupture and luteinization during ex vivo mammalian ovulation

EJ Zaniker, J Zhang, D Russo, R Huang… - Communications …, 2024 - nature.com
During ovulation, the apical wall of the preovulatory follicle breaks down to facilitate gamete
release. In parallel, the residual follicle wall differentiates into a progesterone-producing …

Dietary phytosterols induce infertility in female mice via epigenomic modulations

Y Yamanashi, T Komine, Y Hirota, H Suzuki… - Communications …, 2024 - nature.com
Dietary modifications to overcome infertility have attracted attention; however, scientifically
substantiated information on specific dietary components affecting fertility and their …

[HTML][HTML] 3D culture of ovarian follicles in granular and nanofibrillar hydrogels

M Mihajlovic, DK Pásztor-Jánoska, J Cadenas… - Biomaterials …, 2024 - Elsevier
Abstract 3D culture of ovarian follicles in hydrogel matrices is an important emerging tool for
basic scientific studies as well as clinical applications such as fertility preservation. For …

[PDF][PDF] In Vitro Growth of Mammalian Follicles and Oocytes

K Sakaguchi - Animals, 2024 - mdpi.com
Mammalian ovaries contain a large number of immature follicles, most of which are destined
to degenerate before ovulation [1]. Developing a culture system that enables small oocytes …

[HTML][HTML] A newly improved method of primary cell culture: Tissue block with continuous adhesion subculture in skin fibroblast

Q Deng, L Liu, R Tang, D Xian, J Zhong - Acta Histochemica, 2023 - Elsevier
Abstract Background Fibroblasts (FBs) have been widely used as a typical in vitro cell model
for investigating the biological processes and cell pathophysiological mechanisms …

[HTML][HTML] Gene Expression Analyses in Human Follicles

M Devos, JD Nunes… - JoVE (Journal …, 2023 - www-jove-com-443.vpn.cdutcm.edu …
The ovary is a heterogeneous organ composed of different cell types. To study the molecular
mechanisms occurring during folliculogenesis, the localization of proteins and gene …

Design of a Microphysiologic Ovarian Follicle Culture System Using Multiomics Technology

A Jones - 2023 - deepblue.lib.umich.edu
Life-saving anti-cancer treatments cause irreversible damage to the ovarian follicular
reserve, resulting in infertility for many patients. Development of a translational follicle …