Recent advances in correlative cryo-light and electron microscopy

JA Pierson, JE Yang, ER Wright - Current Opinion in Structural Biology, 2024 - Elsevier
Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging
modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo …

[HTML][HTML] DNA origami signposts for identifying proteins on cell membranes by electron cryotomography

E Silvester, B Vollmer, V Pražák, D Vasishtan… - Cell, 2021 - cell.com
Electron cryotomography (cryoET), an electron cryomicroscopy (cryoEM) modality, has
changed our understanding of biological function by revealing the native molecular details …

Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells

CM Hampton, JD Strauss, Z Ke, RS Dillard… - Nature protocols, 2017 - nature.com
Correlative light and electron microscopy (CLEM) combines spatiotemporal information from
fluorescence light microscopy (fLM) with high-resolution structural data from cryo-electron …

Biological applications at the cutting edge of cryo-electron microscopy

RS Dillard, CM Hampton, JD Strauss… - Microscopy and …, 2018 - academic.oup.com
Cryo-electron microscopy (cryo-EM) is a powerful tool for macromolecular to near-atomic
resolution structure determination in the biological sciences. The specimen is maintained in …

Promotion of virus assembly and organization by the measles virus matrix protein

Z Ke, JD Strauss, CM Hampton, MA Brindley… - Nature …, 2018 - nature.com
Measles virus (MeV) remains a major human pathogen, but there are presently no licensed
antivirals to treat MeV or other paramyxoviruses. Here, we use cryo-electron tomography …

CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy

JE Yang, MR Larson, BS Sibert, S Shrum… - Journal of structural …, 2021 - Elsevier
Cryo-correlative light and electron microscopy (CLEM) is a technique that uses the
spatiotemporal cues from fluorescence light microscopy (FLM) to investigate the high …

Quality assessment of virus-like particles at single particle level: a comparative study

I González-Domínguez, E Puente-Massaguer… - Viruses, 2020 - mdpi.com
Virus-like particles (VLPs) have emerged as a powerful scaffold for antigen presentation and
delivery strategies. Compared to single protein-based therapeutics, quality assessment …

Assembly of respiratory syncytial virus matrix protein lattice and its coordination with fusion glycoprotein trimers

BS Sibert, JY Kim, JE Yang, Z Ke, CC Stobart… - Nature …, 2024 - nature.com
Respiratory syncytial virus (RSV) is an enveloped, filamentous, negative-strand RNA virus
that causes significant respiratory illness worldwide. RSV vaccines are available, however …

The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface

A Presle, S Frémont, A Salles, PH Commere… - Current Biology, 2021 - cell.com
The midbody at the center of the intercellular bridge connecting dividing cells recruits the
machinery essential for the final steps of cytokinesis. 1–5 Successive abscission on both …

Human parainfluenza virus fusion complex glycoproteins imaged in action on authentic viral surfaces

TC Marcink, T Wang, A des Georges, M Porotto… - PLoS …, 2020 - journals.plos.org
Infection by human parainfluenza viruses (HPIVs) causes widespread lower respiratory
diseases, including croup, bronchiolitis, and pneumonia, and there are no vaccines or …