[HTML][HTML] The sec translocase

DJF Du Plessis, N Nouwen, AJM Driessen - Biochimica et Biophysica Acta …, 2011 - Elsevier
The vast majority of proteins trafficking across or into the bacterial cytoplasmic membrane
occur via the translocon. The translocon consists of the SecYEG complex that forms an …

Laser ablation for protein crystal nucleation and seeding

HY Yoshikawa, R Murai, H Adachi… - Chemical Society …, 2014 - pubs.rsc.org
With the recent development in pulsed lasers with ultrashort pulse widths or wavelengths,
spatially precise, low-damage processing by femtosecond or deep-UV laser ablation has …

Structure and function of a membrane component SecDF that enhances protein export

T Tsukazaki, H Mori, Y Echizen, R Ishitani, S Fukai… - Nature, 2011 - nature.com
Protein translocation across the bacterial membrane, mediated by the secretory translocon
SecYEG and the SecA ATPase,,,, is enhanced by proton motive force, and membrane …

Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake

Y Tanaka, K Yoshikaie, A Takeuchi, M Ichikawa… - Science …, 2020 - science.org
We have demonstrated that a bacterial membrane protein, YeeE, mediates thiosulfate
uptake. Thiosulfate is used for cysteine synthesis in bacteria as an inorganic sulfur source in …

[HTML][HTML] Tunnel formation inferred from the I-form structures of the proton-driven protein secretion motor SecDF

A Furukawa, K Yoshikaie, T Mori, H Mori, YV Morimoto… - Cell Reports, 2017 - cell.com
Protein secretion mediated by SecYEG translocon and SecA ATPase is enhanced by
membrane-embedded SecDF by using proton motive force. A previous structural study of …

Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data

IR Krauss, F Sica, CA Mattia, A Merlino - International journal of molecular …, 2012 - mdpi.com
Serum albumin is one of the most widely studied proteins. It is the most abundant protein in
plasma with a typical concentration of 5 g/100 mL and the principal transporter of fatty acids …

Femtosecond laser-induced nucleation of protein in agarose gel

HY Yoshikawa, R Murai, S Sugiyama, G Sazaki… - Journal of crystal …, 2009 - Elsevier
We have performed femtosecond laser-induced nucleation of protein in agarose gel to study
the crystallization dynamics. The use of agarose gel facilitates the monitoring of laser …

Anthracene crystallization induced by single-shot femtosecond laser irradiation: Experimental evidence for the important role of bubbles

K Nakamura, Y Hosokawa, H Masuhara - Crystal growth & design, 2007 - ACS Publications
A mechanism of femtosecond laser-induced crystallization was investigated using a
supersaturated solution of anthracene. When a single-shot femtosecond laser pulse with a …

The TPR domain of BepA is required for productive interaction with substrate proteins and the β‐barrel assembly machinery complex

Y Daimon, C Iwama‐Masui, Y Tanaka… - Molecular …, 2017 - Wiley Online Library
BepA (formerly YfgC) is an Escherichia coli periplasmic protein consisting of an N‐terminal
protease domain and a C‐terminal tetratricopeptide repeat (TPR) domain. We have …

Enhancement of femtosecond laser-induced nucleation of protein in a gel solution

R Murai, HY Yoshikawa, Y Takahashi… - Applied Physics …, 2010 - pubs.aip.org
We found that the use of a gel solution with agarose enhanced femtosecond laser-induced
nucleation and produced hen egg white lysozyme crystals at three to five times lower …