Applications of CRISPR–Cas in agriculture and plant biotechnology

H Zhu, C Li, C Gao - Nature Reviews Molecular Cell Biology, 2020 - nature.com
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology

M Hesami, M Pepe, A Baiton, AMP Jones - Biotechnology Advances, 2023 - Elsevier
For centuries, cannabis has been a rich source of fibrous, pharmaceutical, and recreational
ingredients. Phytocannabinoids are the most important and well-known class of cannabis …

[HTML][HTML] A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy

L Ye, JJ Park, L Peng, Q Yang, RD Chow, MB Dong… - Cell metabolism, 2022 - cell.com
Chimeric antigen receptor (CAR)-T cell-based immunotherapy for cancer and
immunological diseases has made great strides, but it still faces multiple hurdles. Finding …

RNA editing with CRISPR-Cas13

DBT Cox, JS Gootenberg, OO Abudayyeh, B Franklin… - Science, 2017 - science.org
Nucleic acid editing holds promise for treating genetic disease, particularly at the RNA level,
where disease-relevant sequences can be rescued to yield functional protein products. Type …

RNA targeting with CRISPR–Cas13

OO Abudayyeh, JS Gootenberg, P Essletzbichler… - Nature, 2017 - nature.com
RNA has important and diverse roles in biology, but molecular tools to manipulate and
measure it are limited. For example, RNA interference,, can efficiently knockdown RNAs, but …

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

OO Abudayyeh, JS Gootenberg, S Konermann… - Science, 2016 - science.org
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …

Advances in the delivery of RNA therapeutics: from concept to clinical reality

JC Kaczmarek, PS Kowalski, DG Anderson - Genome medicine, 2017 - Springer
The rapid expansion of the available genomic data continues to greatly impact biomedical
science and medicine. Fulfilling the clinical potential of genetic discoveries requires the …

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

J Joung, S Konermann, JS Gootenberg… - Nature protocols, 2017 - nature.com
Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …

Applications of CRISPR technologies in research and beyond

R Barrangou, JA Doudna - Nature biotechnology, 2016 - nature.com
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

DD Kocak, EA Josephs, V Bhandarkar, SS Adkar… - Nature …, 2019 - nature.com
CRISPR (clustered regularly interspaced short palindromic repeat) systems have been
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …