Life with 6000 genes
The genome of the yeast Saccharomyces cerevisiae has been completely sequenced
through a worldwide collaboration. The sequence of 12,068 kilobases defines 5885 …
through a worldwide collaboration. The sequence of 12,068 kilobases defines 5885 …
Protein-protein interactions: methods for detection and analysis
EM Phizicky, S Fields - Microbiological reviews, 1995 - Am Soc Microbiol
The function and activity of a protein are often modulated by other proteins with which it
interacts. This review is intended as a practical guide to the analysis of such protein-protein …
interacts. This review is intended as a practical guide to the analysis of such protein-protein …
An ER-mitochondria tethering complex revealed by a synthetic biology screen
B Kornmann, E Currie, SR Collins, M Schuldiner… - science, 2009 - science.org
Communication between organelles is an important feature of all eukaryotic cells. To
uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we …
uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we …
New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
RD Gietz, S Akio - Gene, 1988 - Elsevier
We describe the production of new alleles of the LEU2 URA3 and TRP1 genes of
Saccharomyces cerevisiae by in vitro mutagenesis. Each new allele, which lacks restriction …
Saccharomyces cerevisiae by in vitro mutagenesis. Each new allele, which lacks restriction …
A microhomology-mediated break-induced replication model for the origin of human copy number variation
Chromosome structural changes with nonrecurrent endpoints associated with genomic
disorders offer windows into the mechanism of origin of copy number variation (CNV). A …
disorders offer windows into the mechanism of origin of copy number variation (CNV). A …
A mutant with a defect in telomere elongation leads to senescence in yeast
V Lundblad, JW Szostak - Cell, 1989 - cell.com
We describe a general assay designed to detect mutants of yeast that are defective for any
of several aspects of telomere function. Using this assay, we have isolated a mutant that …
of several aspects of telomere function. Using this assay, we have isolated a mutant that …
The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability.
CB Brachmann, JM Sherman, SE Devine… - Genes & …, 1995 - genesdev.cshlp.org
Genomic silencing is a fundamental mechanism of transcriptional regulation, yet little is
known about conserved mechanisms of silencing. We report here the discovery of four …
known about conserved mechanisms of silencing. We report here the discovery of four …
Aneuploidy and cancer
H Rajagopalan, C Lengauer - Nature, 2004 - nature.com
In contrast to normal cells, aneuploidy—alterations in the number of chromosomes—is
consistently observed in virtually all cancers. A growing body of evidence suggests that …
consistently observed in virtually all cancers. A growing body of evidence suggests that …
Plasmid construction by homologous recombination in yeast
H Ma, S Kunes, PJ Schatz, D Botstein - Gene, 1987 - Elsevier
We describe a convenient method for constructing new plasmids that relies on interchanging
parts of plasmids by homologous recombination in Saccharomyces cerevisiae. A circular …
parts of plasmids by homologous recombination in Saccharomyces cerevisiae. A circular …
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors
DT Burke, GF Carle, MV Olson - Science, 1987 - science.org
Fragments of exogenous DNA that range in size up to several hundred kilobase pairs have
been cloned into yeast by ligating them to vector sequences that allow their propagation as …
been cloned into yeast by ligating them to vector sequences that allow their propagation as …