Cell-extracellular matrix interactions have been studied extensively using cells cultured in
vitro. These studies indicate that focal adhesion (FA)-based cell-extracellular matrix …

During animal embryogenesis, homeostasis and disease, tissues push and pull on their
surroundings to move forward. Although the force-generating machinery is known, it is …

Zebrafish (Danio rerio) are aquatic vertebrates with significant homology to their terrestrial
counterparts. While zebrafish have a centuries-long track record in developmental and …

The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins
by homology-directed repair in a variety of species. Despite its revolutionary success, there …

The formation and organization of complex blood vessel networks rely on various
biophysical forces, yet the mechanisms governing endothelial cell-cell interactions under …

An essential process during Danio rerio's left-right organizer (Kupffer's Vesicle, KV)
formation is the formation of a motile cilium by developing KV cells which extends into the …

Background Genome editing using CRISPR/Cas9 has become a powerful tool in zebrafish
to generate targeted gene knockouts models. However, its use for targeted knock-in remains …

Site-directed insertion is a powerful approach for generating mutant alleles, but low
efficiency and the need for customisation for each target has limited its application. To …

De novo lumen formation necessitates the precise segregation of junctional proteins from
apical surfaces, yet the underlying mechanisms remain unclear. Using a zebrafish model …

Here, we devised a cloning-free 3′ knock-in strategy for zebrafish using PCR amplified
dsDNA donors that avoids disrupting the targeted genes. The dsDNA donors carry genetic …