Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Cell type-specific gene expression patterns and dynamics during development or in disease
are controlled by cis-regulatory elements (CREs), such as promoters and enhancers …

Mapping single-cell sequencing profiles to comprehensive reference datasets provides a
powerful alternative to unsupervised analysis. However, most reference datasets are …

Cancer-associated fibroblasts (CAFs) are the predominant components of the tumor
microenvironment (TME) and influence cancer hallmarks, but without systematic …

Tumor-infiltrating T cells offer a promising avenue for cancer treatment, yet their states
remain to be fully characterized. Here we present a single-cell atlas of T cells from 308,048 …

Breast cancers are complex cellular ecosystems where heterotypic interactions play central
roles in disease progression and response to therapy. However, our knowledge of their …

Although cellular senescence drives multiple age-related co-morbidities through the
senescence-associated secretory phenotype, in vivo senescent cell identification remains …

Pathological hallmarks of Alzheimer's disease (AD) precede clinical symptoms by years,
indicating a period of cognitive resilience before the onset of dementia. Here, we report that …

Large single-cell atlases are now routinely generated to serve as references for analysis of
smaller-scale studies. Yet learning from reference data is complicated by batch effects …

Liver metastasis, the leading cause of colorectal cancer mortality, exhibits a highly
heterogeneous and suppressive immune microenvironment. Here, we sequenced 97 …