Although the first ancient DNA molecules were extracted more than three decades ago, the
first ancient nuclear genomes could only be characterized after high-throughput sequencing …

The microbiota—the collection of microorganisms that live within and on all mammals—
provides crucial signals for the development and function of the immune system. Increased …

High-throughput techniques based on restriction site-associated DNA sequencing (RADseq)
are enabling the low-cost discovery and genotyping of thousands of genetic markers for any …

Due to postmortem DNA degradation and microbial colonization, most ancient genomes
have low depth of coverage, hindering genotype calling. Genotype imputation can improve …

It has been shown that Neanderthals contributed genetically to modern humans outside
Africa 47,000–65,000 years ago. Here we analyse the genomes of a Neanderthal and a …

It has been shown that highly fragmented DNA is most efficiently converted into DNA
libraries for sequencing if both strands of the DNA fragments are processed independently …

We present a DNA library preparation method that has allowed us to reconstruct a high-
coverage (30×) genome sequence of a Denisovan, an extinct relative of Neandertals. The …

Over the past decade, a genomics revolution, made possible through the development of
high-throughput sequencing, has triggered considerable progress in the study of ancient …

Indigenous populations of the Americas experienced high mortality rates during the early
contact period as a result of infectious diseases, many of which were introduced by …

Ancient DNA is typically highly degraded with appreciable cytosine deamination, and
contamination with present-day DNA often complicates the identification of endogenous …