Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection

AP Demchenko - Methods and applications in fluorescence, 2020 - iopscience.iop.org
Photochemical stability is one of the most important parameters that determine the
usefulness of organic dyes in different applications. This Review addresses key factors that …

Lens-based fluorescence nanoscopy

C Eggeling, KI Willig, SJ Sahl, SW Hell - Quarterly reviews of …, 2015 - cambridge.org
The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …

Degradation kinetics of indocyanine green in aqueous solution

V Saxena, M Sadoqi, J Shao - Journal of pharmaceutical …, 2003 - Wiley Online Library
The degradation kinetics of a near‐infrared fluorescent, diagnostic, and photodynamic
agent, indocyanine green (ICG), was investigated in aqueous solution by steady‐state …

Major signal increase in fluorescence microscopy through dark-state relaxation

G Donnert, C Eggeling, SW Hell - Nature methods, 2007 - nature.com
We report a substantial signal gain in fluorescence microscopy by ensuring that transient
molecular dark states with lifetimes> 1 μs, such as the triplet state relax between two …

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy

J Widengren, A Chmyrov, C Eggeling… - The journal of …, 2007 - ACS Publications
Given the particular importance of dye photostability for single-molecule and fluorescence
fluctuation spectroscopy investigations, refined strategies were explored for how to …

The bright future of single-molecule fluorescence imaging

MF Juette, DS Terry, MR Wasserman, Z Zhou… - Current opinion in …, 2014 - Elsevier
Highlights•The potential impact of multi-color single-molecule FRET imaging.•Key
requirement: ultra-stable fluorophores spanning the visible spectrum.•Intramolecularly …

Taming fluorescent dyes with cucurbituril

WM Nau, J Mohanty - International Journal of Photoenergy, 2005 - Wiley Online Library
The potential of the supramolecular host molecule cucurbit [7] uril to serve as a stabilizing
additive and enhancement agent was investigated for the following dyes in aqueous …

Short tetracysteine tags to β-tubulin demonstrate the significance of small labels for live cell imaging

M Andresen, R Schmitz-Salue… - Molecular biology of the …, 2004 - Am Soc Cell Biol
Genetically encoded tags are of fundamental importance for live cell imaging. We show that
small tetracysteine (TetCys) tags can be highly advantageous for the functionality of the host …

Breaching the Fortress: Photochemistry of DNA-Caged Ag106+

CJ Setzler, CA Arrington, D Lewis… - The Journal of Physical …, 2023 - ACS Publications
A DNA strand can encapsulate a silver molecule to create a nanoscale, aqueous stable
chromophore. A protected cluster that strongly fluoresces can also be weakly photolabile …

Highly photostable and fluorescent microporous solids prepared via solid-state entrapment of boron dipyrromethene dyes in a nascent metal–organic framework

V Glembockyte, M Frenette, C Mottillo… - Journal of the …, 2018 - ACS Publications
We report a strategy to synthesize highly emissive, photostable, microporous materials by
solid-state entrapment of boron dipyrromethene (BODIPY) fluorophores in a metal–organic …