Oligosaccharide synthesis and translational innovation

L Krasnova, CH Wong - Journal of the American Chemical Society, 2019 - ACS Publications
The translation of biological glycosylation in humans to the clinical applications involves
systematic studies using homogeneous samples of oligosaccharides and glycoconjugates …

Enzymatic and cell factory approaches to the production of human milk oligosaccharides

M Faijes, M Castejón-Vilatersana, C Val-Cid… - Biotechnology …, 2019 - Elsevier
Infant formula milk companies try to develop fortified formula milk that mimics human milk as
closely as possible, since it is well-known that breast milk has considerable implications in …

Leloir glycosyltransferases as biocatalysts for chemical production

B Nidetzky, A Gutmann, C Zhong - ACS Catalysis, 2018 - ACS Publications
Glycosylation is a chemical transformation that is centrally important in all glycoscience and
related technologies. Catalysts offering good control over reactivity and selectivity in …

Enabling chemoenzymatic strategies and enzymes for synthesizing sialyl glycans and sialyl glycoconjugates

X Chen - Accounts of Chemical Research, 2023 - ACS Publications
Conspectus Sialic acids are fascinating negatively charged nine-carbon monosaccharides.
Sialic acid-containing glycans and glycoconjugates are structurally diverse, functionally …

Enzyme-Sialylation-Controlled Chemical Sulfation of Glycan Epitopes for Decoding the Binding of Siglec Ligands

S Ma, P Zhang, J Ye, Y Tian, X Tian… - Journal of the …, 2024 - ACS Publications
Widely distributed in nature, sulfated glycan epitopes play important roles in diverse
pathophysiological processes. However, due to their structural complexity, the preparation of …

Exploiting Substrate Specificities of 6-O-Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides

Y Wu, GM Vos, C Huang, D Chapla, ALM Kimpel… - JACS Au, 2023 - ACS Publications
Keratan sulfate (KS) is a glycosaminoglycan that is widely expressed in the extracellular
matrix of various tissue types, where it is involved in many biological processes. Herein, we …

Synthesis of N-Glycolylneuraminic Acid (Neu5Gc) and Its Glycosides

AS Kooner, H Yu, X Chen - Frontiers in immunology, 2019 - frontiersin.org
Sialic acids constitute a family of negatively charged structurally diverse monosaccharides
that are commonly presented on the termini of glycans in higher animals and some …

A Biomimetic Synthetic Strategy Can Provide Keratan Sulfate I and II Oligosaccharides with Diverse Fucosylation and Sulfation Patterns

Y Wu, GP Bosman, D Chapla, C Huang… - Journal of the …, 2024 - ACS Publications
Keratan sulfate (KS) is a proteoglycan that is widely expressed in the extracellular matrix of
various tissue types, where it performs multiple biological functions. KS is the least …

[HTML][HTML] Bacterial sialyltransferases and their use in biocatalytic cascades for sialo-oligosaccharide production

S Schelch, C Zhong, B Petschacher, B Nidetzky - Biotechnology Advances, 2020 - Elsevier
Sialic acids are important recognition sites in protein-and lipid-linked glycans of higher
organisms and of select bacteria and protozoa. They are also prominent in human milk …

H. pylori α1–3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides

H Yu, Y Li, Z Wu, L Li, J Zeng, C Zhao, Y Wu… - Chemical …, 2017 - pubs.rsc.org
Helicobacter pylori α1–3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli
at a level of 30 mg L− 1 culture and used as a diverse catalyst in a one-pot multienzyme …